| http://purl.uniprot.org/citations/15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15187164 | http://www.w3.org/2000/01/rdf-schema#comment | "The beta isoform of the type II regulatory subunit (RIIbeta) of protein kinase A suppresses CREB transcriptional activity and c-Fos production in T cells following activation via the TCR. Because CREB is an integral nuclear transcription factor for IL-2 production by T cells, we tested the hypothesis that RIIbeta down-regulates IL-2 expression and IL-2 production in T cells. Stable transfection of RIIbeta in Jurkat T cells led to an approximately 90% reduction in IL-2 mRNA and IL-2 protein following T cell activation. The inhibition of IL-2 production was associated with phosphorylation of the RIIbeta subunit at serine 114 (pRIIbeta) and localization of pRIIbeta in intranuclear clusters. A serine 114 phosphorylation-defective mutant, RIIbeta(S114A), did not form these intranuclear clusters as well as wild-type RIIbeta, and did not inhibit IL-2 mRNA and protein synthesis, indicating that serine 114 phosphorylation is required for both nuclear localization and down-regulation of IL-2 production by RIIbeta. In contrast to its effect on IL-2, RIIbeta induced constitutive up-regulation of CD154 mRNA and cell surface expression. Thus, pRIIbeta differentially regulates gene expression following T cell activation. Unexpectedly, we also found that stable overexpression of another protein kinase A regulatory subunit, RIalpha, had the opposite effect on IL-2 expression, causing a 3-to 4-fold increase in IL-2 production following stimulation. In summary, our data demonstrate a novel mechanism by which serine 114 phosphorylation and nuclear localization of RIIbeta controls the regulation of gene expression in T cells."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.172.12.7804"xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Tsokos G.C."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Elliott M.R."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Shanks R.A."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Brooks J.W."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Khan I.U."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Nelson B.J."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Juang Y.T."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Kammer G.M."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Burkett P.J."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/author | "Kyttaris V."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/pages | "7804-7812"xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/title | "Down-regulation of IL-2 production in T lymphocytes by phosphorylated protein kinase A-RIIbeta."xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://purl.uniprot.org/core/volume | "172"xsd:string |
| http://purl.uniprot.org/citations/15187164 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15187164 |
| http://purl.uniprot.org/citations/15187164 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15187164 |
| http://purl.uniprot.org/uniprot/#_A3R6R8-mappedCitation-15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15187164 |
| http://purl.uniprot.org/uniprot/#_B3KY43-mappedCitation-15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15187164 |
| http://purl.uniprot.org/uniprot/#_Q13169-mappedCitation-15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15187164 |
| http://purl.uniprot.org/uniprot/#_Q71V48-mappedCitation-15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15187164 |
| http://purl.uniprot.org/uniprot/#_Q6QWN0-mappedCitation-15187164 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15187164 |