| http://purl.uniprot.org/citations/15231838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15231838 | http://www.w3.org/2000/01/rdf-schema#comment | "Here we report the functional characterization of Pwp2, an evolutionary conserved component of the 90 S pre-ribosome. Conditional depletion of the Pwp2 protein in yeast specifically impairs pre-rRNA proccessing at sites A(0), A(1), and A(2), leading to a strong decrease in 18 S rRNA and 40 S ribosomal subunit levels. Pre-ribosomal particle sedimentation analysis indicated that these defects are caused by a block in the formation of 90 S pre-ribosomes. We demonstrate that in Pwp2-depleted cells the U3 small nucleolar ribonucleoprotein is not able to interact with the 35 S pre-rRNA and accumulates as a free complex. Similarly, other 90 S particle components such as Imp3 and Imp4 do not associate with the pre-rRNA precursor in the absence of Pwp2. In addition, we have found that after blocking U3 ribonucleoprotein assembly, Pwp2 predominantly accumulates as a complex in association with five proteins: Dip2, Utp6, Utp13, Utp18, and Utp21. Immunoprecipitation and gradient sedimentation analysis revealed that this Pwp2 small subcomplex is capable of interacting directly with the 35 S pre-rRNA 5' end. Taken together, these results indicate that Pwp2 forms part of a stable particle subunit independent of the U3 small nucleolar ribonucleoprotein that is essential for the initial assembly steps of the 90 S pre-ribosome."xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m404909200"xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/author | "Dosil M."xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/author | "Bustelo X.R."xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/pages | "37385-37397"xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/title | "Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle."xsd:string |
| http://purl.uniprot.org/citations/15231838 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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