http://purl.uniprot.org/citations/15237192 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15237192 | http://www.w3.org/2000/01/rdf-schema#comment | "In previous studies, the distribution of thyrotropes in the chicken pituitary gland has been analyzed by immunohistochemistry using heterologous antibodies. In this study, we examined the distribution of thyroid-stimulating hormone beta subunit-immunopositive (TSHbeta-ip) cells and the expression of TSHbeta mRNA in the pituitary glands of chicken embryos by immunohistochemistry using a specific antiserum to the chicken TSHbeta, in situ hybridization and RT-PCR. Immunohistochemical and morphometric analyses revealed that the TSHbeta-ip cells first appeared on embryonic day 10 (E10) in the pituitary gland and were mainly distributed in the cephalic lobe and that the cell density on E20 was almost 4 times greater than that on E10. The chicken TSHbeta-ip cells could be classified into two types based on morphological characteristics: round-shaped cells and club-shaped cells, which have long cytoplasmic processes. In situ hybridization analysis revealed that TSHbeta mRNA-expressing cells were expressed from E9 in the cephalic lobe and that the extent of TSHbeta mRNA-expressing cells coincided with that of TSHbeta-ip cells. RT-PCR also showed that TSHbeta mRNA was expressed from E9 and that Pit-1 mRNA was expressed from E5. These results clearly demonstrated that the expression of chicken TSHbeta mRNA starts on E9, that TSHbeta-ip cells appear on E10, mainly in the cephalic lobe, and that TSHbeta-ip cells can be classified into two cell types (round- and club-shaped cells)."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.org/dc/terms/identifier | "doi:10.1159/000078424"xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Inoue K."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Nakamura K."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Zheng J."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Sakai T."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Kidokoro H."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Iwasawa A."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Maseki Y."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/author | "Komoda M."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/name | "Cells Tissues Organs"xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/pages | "21-28"xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/title | "Development of thyroid-stimulating hormone beta subunit-producing cells in the chicken embryonic pituitary gland."xsd:string |
http://purl.uniprot.org/citations/15237192 | http://purl.uniprot.org/core/volume | "177"xsd:string |
http://purl.uniprot.org/citations/15237192 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15237192 |
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