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http://purl.uniprot.org/citations/15242859http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15242859http://www.w3.org/2000/01/rdf-schema#comment"

Objective

Diabetes-induced dyslipidemia is seen in streptozotocin-induced diabetic rats. This is caused, in part, by elevated intestinal acyl-coenzyme A:cholesterol acyltransferase (ACAT) activity. Because two ACAT isozymes (ACAT-1 and ACAT-2) were identified, in the present study we determined which ACAT isozyme was involved in the elevated intestinal ACAT activity in diabetic rats.

Methods and results

We cloned a full-length cDNA of rat ACAT-2. Its overexpression in ACAT-deficient AC29 cells demonstrated that the ACAT activity is derived from the cloned cDNA, and a 45-kDa protein of rat ACAT-2 cross-reacts with an anti-human ACAT-2 antibody. The tissue distribution of rat ACAT-2 mRNA revealed its restricted expression to liver and small intestine. Immunohistochemical analyses using an anti-human ACAT-2 antibody demonstrated that ACAT-2 is localized in villus-crypt axis of rat small intestine. The intestinal ACAT activity in diabetic rats was significantly immunodepleted by an anti-ACAT-2 antibody but not by an anti-ACAT-1 antibody. Finally, intestinal ACAT-2 in diabetic rats significantly increased at both protein and mRNA levels as compared with that in control rats.

Conclusions

Our data demonstrate that ACAT-2 isozyme is responsible for the increased intestinal ACAT activity of diabetic rats, suggesting an important role of ACAT-2 for dyslipidemia in diabetic patients. Diabetic rats exhibit dyslipidemia caused, in part, by elevated intestinal acyl-coenzyme A:cholesterol acyltransferase (ACAT) activity. We determined which ACAT isozyme (ACAT-1 or ACAT-2) was involved in the elevated intestinal ACAT activity in diabetic rats. We demonstrated an important role of ACAT-2, implicating its involvement in dyslipidemia in diabetic patients."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.org/dc/terms/identifier"doi:10.1161/01.atv.0000137976.88533.13"xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Furukawa K."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Sasaki Y."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Takeya M."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Sakamoto Y."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Miyazaki A."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Hori M."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Satoh M."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Horiuchi S."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Komohara Y."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/author"Hakamata H."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/name"Arterioscler Thromb Vasc Biol"xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/pages"1689-1695"xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/title"Acyl-coenzyme A:cholesterol acyltransferase-2 (ACAT-2) is responsible for elevated intestinal ACAT activity in diabetic rats."xsd:string
http://purl.uniprot.org/citations/15242859http://purl.uniprot.org/core/volume"24"xsd:string
http://purl.uniprot.org/citations/15242859http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15242859
http://purl.uniprot.org/citations/15242859http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15242859
http://purl.uniprot.org/uniprot/#_G3V7I6-mappedCitation-15242859http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15242859
http://purl.uniprot.org/uniprot/#_Q7TQM4-mappedCitation-15242859http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/15242859
http://purl.uniprot.org/uniprot/G3V7I6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/15242859
http://purl.uniprot.org/uniprot/Q7TQM4http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/15242859