| http://purl.uniprot.org/citations/15254266 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15254266 | http://www.w3.org/2000/01/rdf-schema#comment | "Organization of lipids into membrane microdomains is a vital mechanism of protein processing. Here we show that overexpression of ERG6, a gene involved in ergosterol synthesis, elevates sterol levels 1.5-fold on the vacuole membrane and enhances their homotypic fusion. The mechanism of sterol-enhanced fusion is not via more efficient sorting, but instead promotes increased kinetics of fusion subreactions. We initially isolated ERG6 as a suppressor of a vrp1Delta growth defect selective for vacuole function. VRP1 encodes verprolin, an actin-binding protein that colocalizes to vacuoles. The vrp1Delta mutant has fragmented vacuoles in vivo and isolated vacuoles do not fuse in vitro, indicative of a Vrp1p requirement for membrane fusion. ERG6 overexpression rescues vrp1Delta vacuole fusion in a cytosol-dependent manner. Cytosol prepared from the vrp1Delta strain remains active; therefore, cytosol is not resupplying Vrp1p. Las17p (Vrp1p functional partner) antibodies, which inhibit wild-type vacuole fusion, do not inhibit the fusion of vacuoles from the vrp1Delta-ERG6 overexpression strain. Vacuole-associated actin turnover is decreased in the vrp1Delta strain, but recovered by ERG6 overexpression linking sterol enrichment to actin remodeling. Therefore, the Vrp1p/Las17p requirement for membrane fusion is bypassed by increased sterols, which promotes actin remodeling as part the membrane fusion mechanism."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e04-03-0194"xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/author | "Lehner R."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/author | "Eitzen G."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/author | "Tedrick K."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/author | "Trischuk T."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/pages | "4609-4621"xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/title | "Enhanced membrane fusion in sterol-enriched vacuoles bypasses the Vrp1p requirement."xsd:string |
| http://purl.uniprot.org/citations/15254266 | http://purl.uniprot.org/core/volume | "15"xsd:string |
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