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| Subject | Predicate | Object |
|---|---|---|
| http://purl.uniprot.org/citations/15265687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15265687 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15265687 | http://www.w3.org/2000/01/rdf-schema#comment | "The molecular basis by which proteins are transported along cytoskeletal tracts from the trans-Golgi network (TGN) to the cell periphery remains poorly understood. Previously, using human autoimmune sera, we identified and characterized a TGN protein, p230/Golgin-245, an extensively coiled-coil protein with flexible amino- and carboxyl-terminal ends, that is anchored to TGN membranes and TGN-derived vesicles by its carboxyl-terminal GRIP domain. To identify molecules that interact with the flexible amino-terminal end of p230, we used this domain as bait to screen a human brain cDNA library in a yeast two-hybrid assay. We found that this domain interacts with the carboxyl-terminal domain of MACF1, a protein that cross-links microtubules to the actin cytoskeleton. The interaction was confirmed by co-immunoprecipitation, an in vitro binding assay, double immunofluorescence images demonstrating overlapped localization in HeLa cells, and co-localization of FLAG-tagged constructs containing the interacting domains of these two proteins with their endogenous partners. Expression in HeLa cells of FLAG-tagged constructs containing the interacting domains of p230 and MACF1 disrupted transport of the glycosyl phosphatidyl inositol-anchored marker protein conjugated with yellow fluorescent protein (YFP-SP-GPI), while trafficking of the transmembrane marker protein, vesicular stomatitis virus glycoprotein conjugated with YFP (VSVG3-GL-YFP), was unaffected. Our results suggest that p230, through its interaction with MACF1, provides the molecular link for transport of GPI-anchored proteins along the microtubule and actin cytoskeleton from the TGN to the cell periphery."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2004.04.047"xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2004.04.047"xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Nakamura T."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Nakamura T."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Toh B.H."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Toh B.H."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Ichikawa H."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Ichikawa H."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Tsukada Y."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Tsukada Y."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Kakinuma T."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/author | "Kakinuma T."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/name | "Exp. Cell Res."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/pages | "388-398"xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/pages | "388-398"xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/title | "Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/title | "Interaction between p230 and MACF1 is associated with transport of a glycosyl phosphatidyl inositol-anchored protein from the Golgi to the cell periphery."xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/volume | "298"xsd:string |
| http://purl.uniprot.org/citations/15265687 | http://purl.uniprot.org/core/volume | "298"xsd:string |