| http://purl.uniprot.org/citations/15269838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15269838 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15269838 | http://www.w3.org/2000/01/rdf-schema#comment | "The anti-angiogenic properties of the histidine-proline-rich (H/P) domain of HPRG have recently been described (Juarez JC, et al. Cancer Research 2002; 62: 5344-50). However, the binding site that mediates these properties is unknown. HPRG is evolutionarily, functionally and structurally related to cleaved high molecular weight kininogen (HKa), an anti-angiogenic polypeptide that stimulates apoptosis of proliferating endothelial cells through binding to cell-surface tropomyosin (Zhang J-C, et al. Proc Natl Acad Sci USA 2002; 99: 12224-9). In this study, we demonstrate that HPRG binds with high affinity to FGF-2-stimulated human umbilical vein endothelial cells (HUVEC) and immobilized tropomyosin in a Zn2+ or pH-dependent manner, and that this interaction is mediated by the H/P domain of HPRG. At least two binding sites for HPRG, tropomyosin and heparan sulfate proteoglycans (HSPs), were identified on the surface of FGF-2-activated endothelial cells. Translocation of tropomyosin to the surface of HUVEC occurred in response to FGF-2, and the anti-angiogenic activity of HPRG in a Matrigel plug model was partially inhibited by soluble tropomyosin. These results suggest that HPRG binds to endothelial cell surface tropomyosin which at least partially mediates the antiangiogenic effects of HPRG."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.org/dc/terms/identifier | "doi:10.1160/th04-02-0073"xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.org/dc/terms/identifier | "doi:10.1160/th04-02-0073"xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Qi X."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Qi X."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Guan X."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Guan X."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Shaw D.E."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Shaw D.E."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Morgan W.T."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Morgan W.T."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Donate F."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Donate F."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Juarez J.C."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Juarez J.C."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Mazar A.P."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Mazar A.P."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "McCrae K.R."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "McCrae K.R."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Shipulina N.V."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/author | "Shipulina N.V."xsd:string |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15269838 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |