| http://purl.uniprot.org/citations/15280662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15280662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15280662 | http://www.w3.org/2000/01/rdf-schema#comment | "The localization of the cyclin-dependent kinase inhibitor p27(kip1) is dependent on the phosphorylation of one of three key amino acid residues: S10, T157 and T198. However, it was unclear whether endogenous p27(kip1) is phosphorylated at T198 in the living cell. In the present work we describe the generation and characterization of a polyclonal antibody able to recognize recombinant, transfected as well as endogenous T198-phosphorylated p27(kip1). Using this antibody, we demonstrate that: (1) endogenous p27(kip1) is phosphorylated at T198 in 4 breast cancer cells lines (MCF7, MDA-MB231, MDA-MB436 and MDA-MB468); (2) T198 phosphorylation is increased in breast cancer cells compared with normal mammary epithelial cells (HMEC); (3) T198-phosphorylated p27(kip1) is exclusively cytoplasmic; (4) T198 phosphorylation is dependent on the activity of the PI3K-PKB/Akt pathway, being it drastically reduced by the pharmacological PI3K inhibitor LY294002 or stimulated by the constitutive activation of PKB/Akt. Finally, in primary human breast carcinomas, cytoplasmic accumulation of T198-phosphorylated p27(kip1) parallels Akt activation. We conclude that in breast cancer cells p27(kip1) is phosphorylated at T198 in a PI3K/Akt dependent manner and that this phosphorylation may contribute to p27(kip1) cytoplasmic mislocalization observed in breast cancer."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Fusco A."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Fusco A."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Califano D."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Califano D."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "De Marco C."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "De Marco C."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Motti M.L."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Motti M.L."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Viglietto G."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/author | "Viglietto G."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/name | "Cell Cycle"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/name | "Cell Cycle"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/pages | "1074-1080"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/pages | "1074-1080"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/title | "Akt-dependent T198 phosphorylation of cyclin-dependent kinase inhibitor p27kip1 in breast cancer."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/title | "Akt-dependent T198 phosphorylation of cyclin-dependent kinase inhibitor p27kip1 in breast cancer."xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://purl.uniprot.org/core/volume | "3"xsd:string |
| http://purl.uniprot.org/citations/15280662 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15280662 |
| http://purl.uniprot.org/citations/15280662 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15280662 |