| http://purl.uniprot.org/citations/15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15284113 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously identified a guanosine triphosphatase (GTPase)-activating protein (GAP) male germ cell Rac GAP (MgcRacGAP) that enhanced interleukin-6 (IL-6)-induced macrophage differentiation of murine M1 leukemia cells. Later, MgcRacGAP was found to play crucial roles in cell division. However, how MgcRacGAP enhanced IL-6-induced differentiation remained elusive. Here we show that MgcRacGAP enhances IL-6-induced differentiation through enhancement of signal transducer and activator of transcription-3 (STAT3) activation. MgcRacGAP, Rac, and STAT3 formed a complex in IL-6-stimulated M1 cells, where MgcRacGAP interacted with Rac1 and STAT3 through its cysteine-rich domain and GAP domain. In reporter assays, the wild-type MgcRacGAP enhanced transcriptional activation of STAT3 while a GAP-domain deletion mutant (DeltaGAP) did not significantly enhance it, suggesting that the GAP domain was required for enhancement of STAT3-dependent transcription. Intriguingly, M1 cells expressing DeltaGAP had no effect on the differentiation signal of IL-6, while forced expression of MgcRacGAP rendered M1 cells hyperresponsive to the IL-6-induced differentiation. Moreover, knockdown of MgcRacGAP by RNA interference profoundly suppressed STAT3 activation, implicating MgcRacGAP in the STAT3-dependent transcription. All together, our data not only reveal an important role for MgcRacGAP in STAT3 activation, but also demonstrate that MgcRacGAP regulates IL-6-induced cellular differentiation in which STAT3 plays a pivotal role."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.org/dc/terms/identifier | "doi:10.1182/blood-2004-03-1066"xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Kawashima T."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Nakajima H."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Kitamura T."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Nosaka T."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Bao Y.C."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Minoshima Y."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Tonozuka Y."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Moon Y."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Tsubono Y."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/author | "Hatori T."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/pages | "3550-3557"xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/title | "A GTPase-activating protein binds STAT3 and is required for IL-6-induced STAT3 activation and for differentiation of a leukemic cell line."xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://purl.uniprot.org/core/volume | "104"xsd:string |
| http://purl.uniprot.org/citations/15284113 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15284113 |
| http://purl.uniprot.org/citations/15284113 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15284113 |
| http://purl.uniprot.org/uniprot/#_A0A7I2V395-mappedCitation-15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15284113 |
| http://purl.uniprot.org/uniprot/#_B2RE34-mappedCitation-15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15284113 |
| http://purl.uniprot.org/uniprot/#_A0A1C9J7U0-mappedCitation-15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15284113 |
| http://purl.uniprot.org/uniprot/#_A0A2X0U4T9-mappedCitation-15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15284113 |
| http://purl.uniprot.org/uniprot/#_B4DVM1-mappedCitation-15284113 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15284113 |