| http://purl.uniprot.org/citations/15292147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15292147 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15292147 | http://www.w3.org/2000/01/rdf-schema#comment | "It is well known that the ykvU-ykvV operon is under the regulation of the sigma(E)-associated RNA polymerase (Esigma(E)). In our study, we observed that ykvV is transcribed together with the upstream ykvU gene by Esigma(E) in the mother cell and monocistronically under Esigma(G) control in the forespore. Interestingly, alternatively expressed ykvV in either the forespore or the mother cell increased the sporulation efficiency in the ykvV background. Studies show that the YkvV protein is a member of the thioredoxin superfamily and also contains a putative Sec-type secretion signal at the N terminus. We observed efficient sporulation in a mutant strain obtained by replacing the putative signal peptide of YkvV with the secretion signal sequence of SleB, indicating that the putative signal sequence is essential for spore formation. These results suggest that YkvV is capable of being transported by the putative Sec-type signal sequence into the space between the double membranes surrounding the forespore. The ability of ykvV expression in either compartment to complement is indeed intriguing and further introduces a new dimension to the genetics of B. subtilis spore formation. Furthermore, electron microscopic observation revealed a defective cortex in the ykvV disruptant. In addition, the expression levels of sigma(K)-directed genes significantly decreased despite normal sigma(G) activity in the ykvV mutant. However, immunoblotting with the anti-sigma(K) antibody showed that pro-sigma(K) was normally processed in the ykvV mutant, indicating that YkvV plays an important role in cortex formation, consistent with recent reports. We therefore propose that ykvV should be renamed spoIVH."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.186.16.5450-5459.2004"xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.186.16.5450-5459.2004"xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Ogasawara N."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Ogasawara N."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Takeuchi M."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Takeuchi M."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Sekiguchi J."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Sekiguchi J."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Kobayashi K."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Imamura D."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/author | "Imamura D."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/pages | "5450-5459"xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/pages | "5450-5459"xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/title | "spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis."xsd:string |
| http://purl.uniprot.org/citations/15292147 | http://purl.uniprot.org/core/title | "spoIVH (ykvV), a requisite cortex formation gene, is expressed in both sporulating compartments of Bacillus subtilis."xsd:string |