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http://purl.uniprot.org/citations/15300177http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objective

The objective of this study was to determine the significance of membrane type 1 matrix metalloproteinase (MT1-MMP) activation of MMP-2 in experimental abdominal aortic aneurysms.

Methods

Rat aortas were perfused with either saline as a control or elastase, and harvested on 2, 4, or 7 days after perfusion (n = 5 per treatment group/day). Aortic MT1-MMP and MMP-2 expression and protein were determined by real time polymerase chain reaction and Western blotting, respectively. Aortic explants were used to measure MMP-2 activity by zymography. Rat aortic smooth muscle cells in vitro were exposed to increasing doses of elastase and analyzed for MT-1 MMP expression.

Results

Aneurysms formed in 80% of the elastase-perfused aortas at 7 days, whereas none formed in the saline-perfused aortas. Significantly increased MT1-MMP expression was observed only on day 4, when levels were 6.5-fold higher in elastase-perfused aortas compared with saline-perfused aortas (P < .01). By day 7, MT1-MMP protein was present only in the elastase-perfused aortas (P = .02). By immunohistochemistry, MT1-MMP was detectable only in the elastase-perfused group at day 7. Cleaved MMP-2 activity (P = .045) was increased in elastase-perfused aortas compared with saline perfused aortas at day 7. In rat aortic smooth muscle cells, MT-1 MMP expression increased in response to elastase (P = .02).

Conclusion

The rodent aortic aneurysm model exhibits upregulation of MT1-MMP expression and protein with subsequent increased conversion of MMP-2 from the latent to the cleaved form."xsd:string
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http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Sinha I."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Eliason J.L."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Stanley J.C."xsd:string
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http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Grigoryants V."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Henke P.K."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Ailawadi G."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Deogracias M.P."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Hannawa K.K."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Roelofs K.J."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Upchurch G.R. Jr."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/author"Bethi S."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/name"Surgery"xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/pages"176-182"xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/title"Early MT-1 MMP expression following elastase exposure is associated with increased cleaved MMP-2 activity in experimental rodent aortic aneurysms."xsd:string
http://purl.uniprot.org/citations/15300177http://purl.uniprot.org/core/volume"136"xsd:string
http://purl.uniprot.org/citations/15300177http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15300177
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