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http://purl.uniprot.org/citations/15300214http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15300214http://www.w3.org/2000/01/rdf-schema#comment"

Background

Pancreatic duodenal homeobox gene-1 (PDX-1) has a dual task as a key regulator in pancreatic organogenesis and in functional maintenance of beta cells in adults. Recent studies have shown a close lineage relationship between the liver and the pancreas. In this study, we analyzed the plasticity of the liver by enforced expression of PDX-1 in streptozotocin (STZ)-treated mice under the condition of hepatic regeneration.

Methods

Replication-deficient adenoviruses were constructed by the cosmid-adenoviral DNA terminal protein complex method. Mice were treated with STZ (200 mg/kg ip), and a 40% partial hepatectomy was performed at day 0. After 24 hours, Ad-pdx-1 or Ad-lacZ 2.0 x 10(9) PFU/body was injected via the tail vain into nontreated (control), STZ-treated, or STZ plus partial hepatectomy (Hx)-treated ICR mice. After 7 and 14 days, expression of PDX-1 and islet hormones was examined by immunohistologic and reverse transcription-polymerase chain reaction analysis. Blood glucose concentrations were measured every 2 days. Immunoreactive insulin (IRI) of serum and liver extract was measured by ELISA.

Results

Most hepatocytes of Ad-pdx-1-infected mice were positive for PDX-1 expression by immunohistochemistry. In nontreated mice, very few cells expressed insulin and other hormones. In contrast, insulin and somatostatin were expressed in STZ-treated mice, and more cells were expressed in STZ plus Hx-treated mice. In addition, other beta-cell markers like GLUT2 and glucokinase were observed. Hyperglycemia was improved in STZ-treated mice and STZ plus Hx-treated mice. IRI of serum and liver extract was increased in STZ-treated mice and STZ plus Hx-treated mice. The insulin positive area of the liver in STZ plus Hx-treated mice was larger than that in nontreated and STZ-treated mice.

Conclusions

Ectopic PDX-1 expression alone may be insufficient to induce insulin-producing cells in the liver. STZ-induced hyperglycemia plus partial hepatectomy that leads to diabetic state and hepatic regeneration may stimulate the transdifferentiation of liver cells into insulin-producing cells."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.org/dc/terms/identifier"doi:10.1016/j.surg.2004.05.024"xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Mori T."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Kawaguchi Y."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Imamura M."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Koizumi M."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Fujimoto K."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Ito D."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Kami K."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Doi R."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Gittes G.K."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Toyoda E."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/author"Tulachan S.S."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/name"Surgery"xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/pages"449-457"xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/title"Hepatic regeneration and enforced PDX-1 expression accelerate transdifferentiation in liver."xsd:string
http://purl.uniprot.org/citations/15300214http://purl.uniprot.org/core/volume"136"xsd:string
http://purl.uniprot.org/citations/15300214http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15300214
http://purl.uniprot.org/citations/15300214http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15300214
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