| http://purl.uniprot.org/citations/15302601 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15302601 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15302601 | http://www.w3.org/2000/01/rdf-schema#comment | "The T-domain transcription factors, Tbx5 and Tbx4, play important roles in vertebrate limb and heart development. To identify interacting and potential Tbx-regulating proteins, we performed a yeast two-hybrid screen with the C-terminal domain of Tbx5 as bait. We identified a new PDZ-LIM protein composed of one N-terminal PDZ and three C-terminal LIM domains, which we named chicken LMP-4. Among the Tbx2, 3, 4, 5 subfamily, we observed exclusive interaction with Tbx5 and Tbx4 proteins. Tbx3 nor Tbx2 can substitute for LMP-4 binding. While chicken LMP-4 associates with Tbx5 or Tbx4, it uses distinct LIM domains to bind to the individual proteins. Subcellular co-localization of LMP-4 and Tbx proteins supports the protein interaction and reveals interference of LMP-4 with Tbx protein distribution, tethering the transcription factors to the cytoskeleton. The protein-protein interaction indicates regulation of Tbx function at the level of transcription factor nuclear localization. During chicken limb and heart development, Tbx5/LMP-4 and Tbx4/LMP-4 are tightly co-expressed in a temporal and spatial manner, suggesting that they operate in the same pathway. Surprisingly, chicken LMP-4 expression domains outside those of Tbx5 in the heart led to the discovery of Tbx4 expression in the outflow tract and the right ventricle of this organ. The Tbx4-expressing cells coincide with those of the recently discovered secondary anterior heart-forming field. The discrete posterior or anterior expression domains in the heart and the exclusive fore- or hindlimb expression of Tbx5 and Tbx4, respectively, suggest common pathways in the heart and limbs. The identification of a new Tbx5/4-specific binding factor further suggests a novel mechanism for Tbx transcription factor regulation in development and disease."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ydbio.2004.05.024"xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ydbio.2004.05.024"xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Krause A."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Krause A."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Law E."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Law E."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Zacharias W."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Zacharias W."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Camarata T."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Camarata T."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Linkhart B."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Linkhart B."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Lischke A."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Lischke A."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Miljan E."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Miljan E."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Simon H.-G."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/author | "Simon H.-G."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/name | "Dev. Biol."xsd:string |
| http://purl.uniprot.org/citations/15302601 | http://purl.uniprot.org/core/name | "Dev. Biol."xsd:string |