| http://purl.uniprot.org/citations/15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15316072 | http://www.w3.org/2000/01/rdf-schema#comment | "In epidermal wounds, precursor laminin 5 (alpha3beta3gamma2) is deposited in the provisional basement membrane (PBM) before other BM components. Precursor laminin 5 contains G4/5 globular domains at the carboxyl terminus of the alpha3 chain. Here, the function of G4/5 was evaluated in deposition of laminin 5. Soluble laminin 5, secreted by keratinocytes in culture, is cleaved by an endogenous protease releasing G4/5. Thrombin, a serum protease, cleaves G4/5 indistinguishably from endogenous protease. Soluble human precursor laminin 5, but not cleaved laminin 5, was bound and deposited by mouse keratinocytes null for mouse alpha3 chain (alpha3-/-MKs). The deposition rescued adhesion and spreading and survival. In a model for PBM assembly, precursor laminin 5 was deposited along fibronectin fibrils at the junction between co-cultures of keratinocytes and fibroblasts. In both models, the deposition of precursor laminin 5 was inhibited by removal of G4/5 with thrombin. To confirm that G4/5 participates in deposition, the human LAMA3A gene was modified to produce alpha3 chains either without or with G4/5 that cannot be cleaved. Both precleaved and noncleavable alpha3 isoforms were expressed in alpha3-/-MKs, where they deposited sufficiently to rescue adhesion via integrins alpha3beta1 and alpha6beta4. Despite this similarity, noncleavable laminin 5 was at least threefold more efficiently deposited than precleaved isoform. We conclude that the G4/5 domain in the alpha3 chain facilitates deposition of precursor laminin 5 into the PBM in epidermal wounds."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01310"xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Miyashita Y."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Ryan M.C."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Brown T.A."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Bhattacharya M."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Carter W.G."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Gil S.G."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Sigle R.O."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Yang T.M."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Olerud J."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/author | "Boutaud A."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/pages | "4481-4494"xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/title | "Globular domains 4/5 of the laminin alpha3 chain mediate deposition of precursor laminin 5."xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/15316072 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15316072 |
| http://purl.uniprot.org/citations/15316072 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15316072 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MSA0-mappedCitation-15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15316072 |
| http://purl.uniprot.org/uniprot/#_A0A0A6YYF2-mappedCitation-15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15316072 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MTS5-mappedCitation-15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15316072 |
| http://purl.uniprot.org/uniprot/#_A2A863-mappedCitation-15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15316072 |
| http://purl.uniprot.org/uniprot/#_A2A864-mappedCitation-15316072 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15316072 |