Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/15322127http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15322127http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15322127http://www.w3.org/2000/01/rdf-schema#comment"Trans-splicing introduces a common 5' 22-nucleotide sequence with an N-2,2,7-trimethylguanosine cap (m (2,2,7)(3)GpppG or TMG-cap) to more than 70% of transcripts in the nematodes Caenorhabditis elegans and Ascaris suum. Using an Ascaris embryo cell-free translation system, we found that the TMG-cap and spliced leader sequence synergistically collaborate to promote efficient translation, whereas addition of either a TMG-cap or spliced leader sequence alone decreased reporter activity. We cloned an A. suum embryo eIF4E homolog and demonstrate that this recombinant protein can bind m(7)G- and TMG-capped mRNAs in cross-linking assays and that binding is enhanced by eIF4G. Both the cap structure and the spliced leader (SL) sequence affect levels of A. suum eIF4E cross-linking to mRNA. Furthermore, the differential binding of eIF4E to a TMG-cap and to trans-spliced and non-trans-spliced RNAs is commensurate with the translational activity of reporter RNAs observed in the cell-free extract. Together, these binding data and translation assays with competitor cap analogs suggest that A. suum eIF4E-3 activity may be sufficient to mediate translation of both trans-spliced and non-trans-spliced mRNAs. Bioinformatic analyses demonstrate the SL sequence tends to trans-splice close to the start codon in a diversity of nematodes. This evolutionary conservation is functionally reflected in the optimal SL to AUG distance for reporter mRNA translation in the cell-free system. Therefore, trans-splicing of the SL1 leader sequence may serve at least two functions in nematodes, generation of an optimal 5'-untranslated region length and a specific sequence context (SL1) for optimal translation of trimethylguanosine capped transcripts."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.org/dc/terms/identifier"doi:10.1074/jbc.M407475200"xsd:string
http://purl.uniprot.org/citations/15322127http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m407475200"xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Lall S."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Lall S."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Darzynkiewicz E."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Darzynkiewicz E."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Stepinski J."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Stepinski J."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Davis R.E."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Davis R.E."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Jankowska-Anyszka M."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Jankowska-Anyszka M."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Friedman C.C."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/author"Friedman C.C."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/name"J Biol Chem"xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/pages"45573-45585"xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/pages"45573-45585"xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/title"Contribution of trans-splicing, 5' -leader length, cap-poly(A) synergism, and initiation factors to nematode translation in an Ascaris suum embryo cell-free system."xsd:string
http://purl.uniprot.org/citations/15322127http://purl.uniprot.org/core/title"Contribution of trans-splicing, 5' -leader length, cap-poly(A) synergism, and initiation factors to nematode translation in an Ascaris suum embryo cell-free system."xsd:string
▶