| http://purl.uniprot.org/citations/15345469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15345469 | http://www.w3.org/2000/01/rdf-schema#comment | "The proteinase-activated thrombin receptor-1 (PAR-1) belongs to a unique family of G protein-coupled receptors activated by proteolytic cleavage. We studied the effect of PAR-1 activation in the regulation of ion transport in mouse colon in vitro. Expression of PAR-1 in mouse colon was assessed by RT-PCR and immunohistochemistry. To study the role of PAR-1 activation in chloride secretion, mouse colon was mounted in Ussing chambers. Changes in short-circuit current (Isc) were measured in tissues exposed to either thrombin, saline, the PAR-1-activating peptide TFLLR-NH2, or the inactive reverse peptide RLLFT-NH2, before electrical field stimulation (EFS). Experiments were repeated in the presence of either a PAR-1 antagonist or in PAR-1-deficient mice to assess receptor specificity. In addition, studies were conducted in the presence of chloride-free buffer or the muscarinic antagonist atropine to assess chloride dependency and the role of cholinergic neurons in the PAR-1-induced effect. PAR-1 mRNA was expressed in full-thickness specimens and mucosal scrapings of mouse colon. PAR-1 immunoreactivity was found on epithelial cells and on neurons in submucosal ganglia where it was colocalized with both VIP and neuropeptide Y. After PAR-1 activation by thrombin or TFLLR-NH2, secretory responses to EFS but not those to forskolin or carbachol were significantly reduced. The reduction in the response to EFS was not observed in the presence of the PAR-1 antagonist, in PAR-1-deficient mice, when chloride was excluded from the bathing medium, or when atropine was present. PAR-1 is expressed in submucosal ganglia in the mouse colon and its activation leads to a decrease in neurally evoked epithelial chloride secretion."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.org/dc/terms/identifier | "doi:10.1152/ajpgi.00112.2004"xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Andrade-Gordon P."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Sharkey K.A."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Hollenberg M.D."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Vergnolle N."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Keenan C.M."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Cirino G."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "MacNaughton W.K."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Buresi M.C."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/author | "Cirillo D."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/name | "Am J Physiol Gastrointest Liver Physiol"xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/pages | "G337-45"xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/title | "Activation of proteinase-activated receptor-1 inhibits neurally evoked chloride secretion in the mouse colon in vitro."xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://purl.uniprot.org/core/volume | "288"xsd:string |
| http://purl.uniprot.org/citations/15345469 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15345469 |
| http://purl.uniprot.org/citations/15345469 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15345469 |
| http://purl.uniprot.org/uniprot/#_P30558-mappedCitation-15345469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15345469 |
| http://purl.uniprot.org/uniprot/#_Q3UK34-mappedCitation-15345469 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15345469 |
| http://purl.uniprot.org/uniprot/Q3UK34 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15345469 |
| http://purl.uniprot.org/uniprot/P30558 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15345469 |