| http://purl.uniprot.org/citations/15355958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15355958 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15355958 | http://www.w3.org/2000/01/rdf-schema#comment | "Human light chain 3/MAP1LC3B, an autophagosomal ortholog of yeast Atg8, is conjugated to phospholipid (PL) via ubiquitylation-like reactions mediated by human Atg7 and Atg3. Since human Atg4B was found to cleave the carboxyl terminus of MAP1LC3B in vitro, we hypothesized that this exposes its carboxyl-terminal Gly(120). It was recently reported, however, that when Myc-MAP1LC3B-His is expressed in HEK293 cells, its carboxyl terminus is not cleaved. (Tanida, I., Sou, Y.-s., Ezaki, J., Minematsu-Ikeguchi, N., Ueno, T., and Kominami, E. (2004) J. Biol. Chem. 279, 36268-36276). To clarify this contradiction, we sought to determine whether the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions. When MAP1LC3B-3xFLAG and Myc-MAP1LC3B-His were expressed in HEK293 cells, their carboxyl termini were cleaved, whereas there was little cleavage of mutant proteins MAP1LC3B(G120A)-3xFLAG and Myc-MAP1LC3B(G120A)-His, containing Ala in place of Gly(120). An in vitro assay showed that Gly(120) is essential for carboxyl-terminal cleavage by human Atg4B as well as for formation of the intermediates Atg7-MAP1LC3B (ubiquitin-activating enzyme-substrate) and Atg3-MAP1LC3B (ubiquitin carrier protein-substrate). Recombinant MAP1LC3B-PL was fractionated into the 100,000 x g pellet in a manner similar to that shown for endogenous MAP1LC3B-PL. RNA interference of MAP1LC3B mRNA resulted in a decrease in both endogenous MAP1LC3B-PL and MAP1LC3B. These results indicate that the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m407016200"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m407016200"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Ueno T."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Ueno T."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Kominami E."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Kominami E."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Tanida I."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/author | "Tanida I."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/pages | "47704-47710"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/pages | "47704-47710"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/title | "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/title | "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes."xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15355958 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15355958 |
| http://purl.uniprot.org/citations/15355958 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15355958 |
| http://purl.uniprot.org/citations/15355958 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15355958 |
| http://purl.uniprot.org/citations/15355958 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15355958 |