| http://purl.uniprot.org/citations/15361071 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15361071 | http://www.w3.org/2000/01/rdf-schema#comment | "HnRNP K (heterogeneous nuclear ribonucleoprotein K) was biochemically purified from a screen of proteins co-purifying with binding activity to the osteocalcin promoter. We identify hnRNP K as a novel repressor of osteocalcin gene transcription. Overexpression of hnRNP K lowers the expression of osteocalcin mRNA by 5-fold. Furthermore, luciferase reporter assays demonstrate that overexpression of hnRNP K represses osteocalcin transcription from a CT (cytosine/thymidine)-rich element in the proximal promoter. Electrophoretic mobility-shift analysis reveals that recombinant hnRNP K binds to the CT-rich element, but binds ss (single-stranded), rather than ds (double-stranded) oligonucleotide probes. Accordingly, hnRNP K antibody can supershift a binding activity present in nuclear extracts using ss sense, but not antisense or ds oligonucleotides corresponding to the CT-rich -95 to -47 osteocalcin promoter. Importantly, addition of recombinant hnRNP K to ROS 17/2.8 nuclear extract disrupts formation of a DNA-protein complex on ds CT element oligonucleotides. This action is mutually exclusive with hnRNP K's ability to bind ss DNA. These results demonstrate that hnRNPK, although co-purified with a dsDNA-binding activity, does not itself bind dsDNA. Rather, hnRNP K represses osteocalcin gene transcription by inhibiting the formation of a transcriptional complex on the CT element of the osteocalcin promoter."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20040680"xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/author | "Towler D.A."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/author | "Civitelli R."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/author | "Lecanda F."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/author | "Stains J.P."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/pages | "613-623"xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/title | "Heterogeneous nuclear ribonucleoprotein K represses transcription from a cytosine/thymidine-rich element in the osteocalcin promoter."xsd:string |
| http://purl.uniprot.org/citations/15361071 | http://purl.uniprot.org/core/volume | "385"xsd:string |
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