http://purl.uniprot.org/citations/15369763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/15369763 | http://www.w3.org/2000/01/rdf-schema#comment | "We report a novel modification of spliceosome proteins Sm D1, Sm D3, and Sm B/B'. L292 mouse fibroblasts were labeled in vivo with [3H]methionine. Sm D1, Sm D3, and Sm B/B' were purified from either nuclear extracts, cytosolic extracts or a cytosolic 6S complex by immunoprecipitation of the Sm protein-containing complexes and then separation by electrophoresis on a polyacrylamide gel containing urea. The isolated Sm D1, Sm D3 or Sm B/B' proteins were hydrolyzed to amino acids and the products were analyzed by high-resolution cation exchange chromatography. Sm D1, Sm D3, and Sm B/B' isolated from nuclear fractions were all found to contain omega-NG-monomethylarginine and symmetric omega-NG,NG'-dimethylarginine, modifications that have been previously described. In addition, Sm D1, Sm D3, and Sm B/B' were also found to contain asymmetric omega-NG,NG-dimethylarginine in these nuclear fractions. Analysis of Sm B/B' from cytosolic fractions and Sm B/B' and Sm D1 from cytosolic 6S complexes showed only the presence of omega-NG-monomethylarginine and symmetric omega-NG,NG'-dimethylarginine. These results indicate that Sm D1, Sm D3, and Sm B/B' are asymmetrically dimethylated and that these modified proteins are located in the nucleus. In reactions in which Sm D1 or Sm D3 was methylated in vitro with a hemagglutinin-tagged PRMT5 purified from HeLa cells, we detected both symmetric omega-NG,NG'-dimethylarginine and asymmetric omega-NG,NG-dimethylarginine when reactions were done in a Tris/HCl buffer, but only detected symmetric omega-NG,NG'-dimethylarginine when a sodium phosphate buffer was used. These results suggest that the activity responsible for the formation of asymmetric dimethylated arginine residues in Sm proteins is either PRMT5 or a protein associated with it in the immunoprecipitated complex."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2004.08.107"xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Lee J.H."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Clarke S."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Cook J.R."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Pestka S."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Miranda T.B."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Gunderson S.I."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Zieve G.W."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/author | "Khusial P."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/pages | "382-387"xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/title | "Spliceosome Sm proteins D1, D3, and B/B' are asymmetrically dimethylated at arginine residues in the nucleus."xsd:string |
http://purl.uniprot.org/citations/15369763 | http://purl.uniprot.org/core/volume | "323"xsd:string |
http://purl.uniprot.org/citations/15369763 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15369763 |
http://purl.uniprot.org/citations/15369763 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15369763 |
http://purl.uniprot.org/uniprot/O14744#attribution-391A35EEF52C9E440F7E60B4F8D62686 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/Q8CIG8#attribution-3638F2DC4B1A48C1F3FE49D476312A23 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/P27048#attribution-391A35EEF52C9E440F7E60B4F8D62686 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/P62320#attribution-391A35EEF52C9E440F7E60B4F8D62686 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/P62315#attribution-391A35EEF52C9E440F7E60B4F8D62686 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/#_A0A0G2JGN4-mappedCitation-15369763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15369763 |
http://purl.uniprot.org/uniprot/#_A0A0R4J049-mappedCitation-15369763 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15369763 |