| http://purl.uniprot.org/citations/15370296 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15370296 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15370296 | http://www.w3.org/2000/01/rdf-schema#comment | "Caveolae are plasmalemmal domains enriched with cholesterol, caveolins, and signaling molecules. Normally, cells that express caveolin-1 also express caveolin-2, but this has not been demonstrated in bovine aortic endothelial cells (BAECs). Here, we show that BAECs express caveolin-2, which localizes in caveolae with caveolin-1. We have cloned the bovine caveolin-2 gene and after comparison with known protein sequences (human, murine, rat, and canine) have found divergent immunogenic regions (amino acid [aa] 21 to aa 50 and aa 79 to 88), which may explain the inability to detect caveolin-2 in different cell types. We developed a bovine caveolin-2-specific antibody to examine this protein's expression and localization in BAECs. We used differential gradient centrifugations and immunoprecipitation to show that bovine caveolin-2 and caveolin-1 form a hetero-oligomer in plasma membrane caveolae. Using immunocytochemistry we show that a pool of caveolin-2 also colocalizes with the cis-Golgi in static culture, but unlike caveolin-1, this Golgi associated pool is maintained after 1 day of shear exposure. Therefore, the interaction of caveolin-2 with caveolin-1 could play an important role in caveolae biogenesis and shear stimulated mechano-signal transduction."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.org/dc/terms/identifier | "doi:10.1080/10623320490512282"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.org/dc/terms/identifier | "doi:10.1080/10623320490512282"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Park H."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Park H."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Jo H."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Jo H."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Boyd N."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Boyd N."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Cherukuri R."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Cherukuri R."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Coleman S."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Coleman S."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Sun W.P."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/author | "Sun W.P."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/name | "Endothelium"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/name | "Endothelium"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/pages | "189-198"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/pages | "189-198"xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/title | "Bovine caveolin-2 cloning and effects of shear stress on its localization in bovine aortic endothelial cells."xsd:string |
| http://purl.uniprot.org/citations/15370296 | http://purl.uniprot.org/core/title | "Bovine caveolin-2 cloning and effects of shear stress on its localization in bovine aortic endothelial cells."xsd:string |