| http://purl.uniprot.org/citations/15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15377665 | http://www.w3.org/2000/01/rdf-schema#comment | "The CCAAT displacement protein/Cut homeobox (CDP/Cux) transcription factor is expressed as multiple isoforms that may contain up to four DNA-binding domains: Cut repeats 1, 2, and 3 (CR1, CR2, CR3) and the Cut homeodomain (HD). The full-length protein, which contains all four DNA-binding domains, is surprisingly less efficient than the shorter isoforms in DNA binding. Using a panel of recombinant proteins expressed in mammalian or bacterial cells, we have identified a domain at the extreme N terminus of the protein that can inhibit DNA binding. This domain was able to inhibit the activity of full-length CDP/Cux and of proteins containing various combinations of DNA-binding domains: CR1CR2, CR3HD, or CR2CR3HD. Since inhibition of DNA binding was also observed with purified proteins obtained from bacteria, we conclude that autoinhibition does not require post-translational modification or interaction with an interacting protein but instead functions through an intramolecular mechanism. Antibodies directed against the N-terminal region were able to partially relieve inhibition. In vivo, the transition between the inactive and active states for DNA binding is likely to be governed by posttranslational modifications and/or interaction with one or more protein partners. In addition, we show that the relief of autoinhibition can be accomplished via the proteolytic processing of CDP/Cux. Altogether, these results reveal a novel mode of regulation that serves to modulate the DNA binding activity of CDP/Cux."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m409484200"xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Berube G."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Leduy L."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Nepveu A."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Truscott M."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/author | "Raynal L."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/pages | "49787-49794"xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/title | "The N-terminal region of the CCAAT displacement protein (CDP)/Cux transcription factor functions as an autoinhibitory domain that modulates DNA binding."xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15377665 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15377665 |
| http://purl.uniprot.org/citations/15377665 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15377665 |
| http://purl.uniprot.org/uniprot/#_P70381-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_P70403-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BJ26-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BJL7-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_E9QKE9-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BLJ7-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BLS0-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BJN3-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |
| http://purl.uniprot.org/uniprot/#_H3BJQ9-mappedCitation-15377665 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15377665 |