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| http://purl.uniprot.org/citations/15380617 | http://www.w3.org/2000/01/rdf-schema#comment | "Chk2 is a key player of the DNA damage signalling pathway. To identify new regulators of this kinase, we performed a yeast two-hybrid screen and found that Chk2 associated with the B' regulatory subunit of protein phosphatase PP2A. In vitro GST-Chk2 pulldowns demonstrated that B'gamma isoforms bound to Chk2 with the strongest apparent affinity. This was confirmed in cellulo by co-immunoprecipitation after overexpression of the respective partners in HEK293 cells. The A and C subunits of PP2A were present in the complexes, suggesting that Chk2 was associated with a functionnal PP2A. In vitro kinase assays showed that B'gamma3 was a potent Chk2 substrate. This phosphorylation increased the catalytic phosphatase activity of PP2A measured on MAP kinase-phosphorylated myelin basic protein as well as on autophosphorylated Chk2. Finally, we demonstrated that overexpressing B'gamma3 in HEK293 suppressed the phosphorylation of Chk2 induced by a genotoxic treatment, suggesting that PP2A may counteract the action of the checkpoint kinase in living cells."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.biolcel.2004.04.010"xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/author | "Dozier C."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/author | "Baricault L."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/author | "Tonasso L."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/author | "Darbon J.M."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/author | "Bonyadi M."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/name | "Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/pages | "509-517"xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/title | "Regulation of Chk2 phosphorylation by interaction with protein phosphatase 2A via its B' regulatory subunit."xsd:string |
| http://purl.uniprot.org/citations/15380617 | http://purl.uniprot.org/core/volume | "96"xsd:string |
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