| http://purl.uniprot.org/citations/15381069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15381069 | http://www.w3.org/2000/01/rdf-schema#comment | "An important step in copper homeostasis is delivery of copper to a specific P-type ATPase in the Golgi apparatus (Ccc2 in yeast, ATP7A and ATP7B in humans) by a small copper chaperone protein (Atx1 in yeast, ATOX1 in humans). Atx1 and ATOX1 both contain an MXCXXC motif that is also present in Ccc2 (two motifs) and ATP7A/B (six motifs). Protein-protein interactions probably require coordination of one Cu(I) by cysteines from both MXCXXC motifs. We applied yeast two-hybrid analysis to screen systematically all possible interactions between MXCXXC-containing domains in these proteins. We demonstrate that ATOX1 and Atx1 preferentially interact with domains 2 and 4 of ATP7B and that Atx1 interacts with both Ccc2 domains. All combinations show a remarkable bell-shaped dependency on copper concentration that is maximal just below normal copper levels. Our results suggest that yeast two-hybrid analysis can be used to study the intracellular copper status of a cell."xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2004.08.160"xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/author | "Klomp L.W."xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/author | "Merkx M."xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/author | "van Dongen E.M."xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/pages | "789-795"xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/title | "Copper-dependent protein-protein interactions studied by yeast two-hybrid analysis."xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://purl.uniprot.org/core/volume | "323"xsd:string |
| http://purl.uniprot.org/citations/15381069 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15381069 |
| http://purl.uniprot.org/citations/15381069 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15381069 |
| http://purl.uniprot.org/uniprot/#_P38995-mappedCitation-15381069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15381069 |
| http://purl.uniprot.org/uniprot/#_P38636-mappedCitation-15381069 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15381069 |
| http://purl.uniprot.org/uniprot/P38995 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15381069 |
| http://purl.uniprot.org/uniprot/P38636 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15381069 |