| http://purl.uniprot.org/citations/15381704 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15381704 | http://www.w3.org/2000/01/rdf-schema#comment | "Choline acetyltransferase (ChAT) synthesizes acetylcholine in cholinergic neurons; regulation of its activity or response to physiological stimuli is poorly understood. We show that ChAT is differentially phosphorylated by protein kinase C (PKC) isoforms on four serines (Ser-440, Ser-346, Ser-347, and Ser-476) and one threonine (Thr-255). This phosphorylation is hierarchical, with phosphorylation at Ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. Ser-476 with Ser-440 and Ser-346/347 maintains basal ChAT activity. Ser-440 is targeted by Arg-442 for phosphorylation by PKC. Arg-442 is mutated spontaneously (R442H) in congenital myasthenic syndrome, rendering ChAT inactive and causing neuromuscular failure. This mutation eliminates phosphorylation of Ser-440, and Arg-442, not phosphorylation of Ser-440, appears primarily responsible for ChAT activity, with Ser-440 phosphorylation modulating catalysis. Finally, basal ChAT phosphorylation in neurons is mediated predominantly by PKC at Ser-476, with PKC activation increasing phosphorylation at Ser-440 and enhancing ChAT activity."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m407085200"xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Doherty-Kirby A."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Brewer D."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Dobransky T."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Rylett R.J."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Kim A.R."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/author | "Lajoie G."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/pages | "52059-52068"xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/title | "Protein kinase C isoforms differentially phosphorylate human choline acetyltransferase regulating its catalytic activity."xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15381704 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15381704 |
| http://purl.uniprot.org/citations/15381704 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15381704 |
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