| http://purl.uniprot.org/citations/15456767 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15456767 | http://www.w3.org/2000/01/rdf-schema#comment | "The collecting duct of normal kidney exhibits significant activity of the MEK1/2-ERK1/2 pathway as shown in vivo by immunostaining of phosphorylated active ERK1/2 (pERK1/2). The MEK1/2-ERK1/2 pathway controls many different ion transports both in proximal and distal nephron, raising the question of whether this pathway is involved in the basal and/or hormone-dependent transepithelial sodium reabsorption in the principal cell of the cortical collecting duct (CCD), a process mediated by the apical epithelial sodium channel and the basolateral sodium pump (Na,K-ATPase). To answer this question we used ex vivo microdissected CCDs from normal mouse kidney or in vitro cultured mpkCCDcl4 principal cells. Significant basal levels of pERK1/2 were observed ex vivo and in vitro. Aldosterone and vasopressin, known to up-regulate sodium reabsorption in CCDs, did not change ERK1/2 activity either ex vivo or in vitro. Basal and aldosterone- or vasopressin-stimulated sodium transport was down-regulated by the MEK1/2 inhibitor PD98059, in parallel with a decrease in pERK1/2 in vitro. The activity of Na,K-ATPase but not that of epithelial sodium channel was inhibited by MEK1/2 inhibitors in both unstimulated and aldosterone- or vasopressin-stimulated CCDs in vitro. Cell surface biotinylation showed that intrinsic activity rather than cell surface expression of Na,K-ATPase was controlled by pERK1/2. PD98059 also significantly inhibited the activity of Na,K-ATPase ex vivo. Our data demonstrate that the ERK1/2 pathway controls Na,K-ATPase activity and transepithelial sodium transport in the principal cell and indicate that basal constitutive activity of the ERK1/2 pathway is a critical component of this control."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m405674200"xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Mercier A."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Schild L."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Horisberger J.D."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Rossier B.C."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Firsov D."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Michlig S."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/author | "Doucet A."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/pages | "51002-51012"xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/title | "ERK1/2 controls Na,K-ATPase activity and transepithelial sodium transport in the principal cell of the cortical collecting duct of the mouse kidney."xsd:string |
| http://purl.uniprot.org/citations/15456767 | http://purl.uniprot.org/core/volume | "279"xsd:string |
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