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http://purl.uniprot.org/citations/15458643http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15458643http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15458643http://www.w3.org/2000/01/rdf-schema#comment"

Background

Proteolytic degradation of mitotic regulatory proteins first requires these targets to be ubiquitinated. This is regulated at the level of conjugation of ubiquitin to substrates by the anaphase-promoting complex/cyclosome (APC/C) ubiquitin-protein ligase. Substrate specificity and temporal activity of the APC/C has been thought to lie primarily with its two activators, Cdc20/Fizzy and Cdh1/Fizzy-related.

Results

Here, we show that reduction in the E2 ubiquitin-conjugating enzyme (UBC) of the E2-C family that is encoded by the Drosophila gene vihar (vih), by either mutation or RNAi, leads to an accumulation of cells in a metaphase-like state. Cyclin B accumulates to high levels in all mitotic vih cells, particularly at the spindle poles. Vihar E2-C is present in the cytoplasm of mitotic cells but also associates with centrosomes, and its own degradation is initiated at the metaphase-anaphase transition. Expression of destruction D box mutants of vihar in the syncytial embryo results in mitotic arrest at late anaphase. In contrast to hypomorphic mutants, Cyclin B is degraded at the spindle poles and accumulates in the equatorial region of the spindle.

Conclusions

In Drosophila, the Vihar E2 UBC contributes to the spatiotemporal control of Cyclin B degradation that first occurs at the spindle poles. APC/C-mediated proteolysis of Vihar E2-C autoinactivates the APC/C at the centrosome before a second wave of proteolysis to degrade Cyclin B on the rest of the spindle and elsewhere in the cell."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2004.09.023"xsd:string
http://purl.uniprot.org/citations/15458643http://purl.org/dc/terms/identifier"doi:10.1016/j.cub.2004.09.023"xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Glover D.M."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Glover D.M."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Kraft C."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Kraft C."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Peters J.-M."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Peters J.-M."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Mathe E."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Mathe E."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Deak P."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Deak P."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Giet R."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/author"Giet R."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/name"Curr. Biol."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/pages"1723-1733"xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/pages"1723-1733"xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/title"The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation."xsd:string
http://purl.uniprot.org/citations/15458643http://purl.uniprot.org/core/title"The E2-C vihar is required for the correct spatiotemporal proteolysis of cyclin B and itself undergoes cyclical degradation."xsd:string
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