| http://purl.uniprot.org/citations/15464988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15464988 | http://www.w3.org/2000/01/rdf-schema#comment | "The sialyl Lewis X (sLe(x)) determinant on leukocytes serves as a ligand for selectin family cell adhesion molecules, and selectin-carbohydrate interaction is considered to play an important role in the process of leukocyte extravasation during inflammation. Among several alpha1-3 fucosyltransferases (FucTs), FucT-VII plays a critical role in the biosynthesis of sLe(x)-epitopes. Therefore, small molecules specifically designed to inhibit the FucT-VII enzyme may have potential as anti-inflammatory agents. Here, we have developed a versatile cell-based assay system to monitor sLe(x) biosynthesis using the GeneSwitch System. This system is a mifepristone (MFP)-inducible mammalian expression system, and human transfectant T lymphoblasts expressed the mRNA of FucT-VII and the sLe(x)-epitopes on the cell surface in a time-dependent manner in the presence of MFP, with very low background transcription. Furthermore, when the transfectants were treated with the FucT-VII inhibitor panosialin, sLe(x) expression on the induced cells was inhibited dose dependently without alteration at the mRNA level of FucT-VII. These results suggest that the FucT-VII may be a major regulator of the biosynthesis of the sLe(x)-epitopes on T lymphoblasts, and this cell-based assay may be utilized for a screening system of FucT-VII inhibitors."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2004.09.025"xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/author | "Sugita T."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/author | "Furuya S."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/author | "Miyashiro M."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/author | "Fujishige K."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/pages | "98-107"xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/title | "Highly sensitive cell-based assay system to monitor the sialyl Lewis X biosynthesis mediated by alpha1-3 fucosyltransferase-VII."xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://purl.uniprot.org/core/volume | "324"xsd:string |
| http://purl.uniprot.org/citations/15464988 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15464988 |
| http://purl.uniprot.org/citations/15464988 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15464988 |
| http://purl.uniprot.org/uniprot/#_Q11130-mappedCitation-15464988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15464988 |
| http://purl.uniprot.org/uniprot/Q11130 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15464988 |