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http://purl.uniprot.org/citations/15466478http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15466478http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15466478http://www.w3.org/2000/01/rdf-schema#comment"The degradation of elaidic acid (9-trans-octadecenoic acid), oleic acid, and stearic acid by rat mitochondria was studied to determine whether the presence of a trans double bond in place of a cis double bond or no double bond affects beta-oxidation. Rat mitochondria from liver or heart effectively degraded the coenzyme A derivatives of all three fatty acids. However, with elaidoyl-CoA as a substrate, a major metabolite accumulated in the mitochondrial matrix. This metabolite was isolated and identified as 5-trans-tetradecenoyl-CoA. In contrast, little or none of the corresponding metabolites were detected with oleoyl-CoA or stearoyl-CoA as substrates. A kinetic study of long-chain acyl-CoA dehydrogenase (LCAD) and very long-chain acyl-CoA dehydrogenase revealed that 5-trans-tetradecenoyl-CoA is a poorer substrate of LCAD than is 5-cis-tetradecenoyl-CoA, while both unsaturated acyl-CoAs are poor substrates of very long-chain acyl-CoA dehydrogenase when compared with myristoyl-CoA. Tetradecenoic acid and tetradecenoylcarnitine were detected by gas chromatography/mass spectrometry and tandem mass spectrometry, respectively, when rat liver mitochondria were incubated with elaidoyl-CoA but not when oleoyl-CoA was the substrate. These observations support the conclusion that 5-trans-tetradecenoyl-CoA accumulates in the mitochondrial matrix, because it is less efficiently dehydrogenated by LCAD than is its cis isomer and that the accumulation of this beta-oxidation intermediate facilitates its hydrolysis and conversion to 5-trans-tetradecenoylcarnitine thereby permitting a partially degraded fatty acid to escape from mitochondria. Analysis of this compromised but functional process provides insight into the operation of beta-oxidation in intact mitochondria."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m409640200"xsd:string
http://purl.uniprot.org/citations/15466478http://purl.org/dc/terms/identifier"doi:10.1074/jbc.m409640200"xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Liang X."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Liang X."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Schulz H."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Schulz H."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Yu W."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Yu W."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Ensenauer R.E."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Ensenauer R.E."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Sweetman L."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Sweetman L."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Vockley J."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/author"Vockley J."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/date"2004"xsd:gYear
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/pages"52160-52167"xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/pages"52160-52167"xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/title"Leaky beta-oxidation of a trans-fatty acid: incomplete beta-oxidation of elaidic acid is due to the accumulation of 5-trans-tetradecenoyl-CoA and its hydrolysis and conversion to 5-trans-tetradecenoylcarnitine in the matrix of rat mitochondria."xsd:string
http://purl.uniprot.org/citations/15466478http://purl.uniprot.org/core/title"Leaky beta-oxidation of a trans-fatty acid: incomplete beta-oxidation of elaidic acid is due to the accumulation of 5-trans-tetradecenoyl-CoA and its hydrolysis and conversion to 5-trans-tetradecenoylcarnitine in the matrix of rat mitochondria."xsd:string
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