| http://purl.uniprot.org/citations/15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15466853 | http://www.w3.org/2000/01/rdf-schema#comment | "P-selectin glycoprotein ligand-1 (PSGL-1), a sialomucin expressed on leukocytes, is a major ligand for P-selectin and mediates leukocyte rolling on the endothelium. Here we show that human PSGL-1 interacts with CCL27 (CTACK/ILC/ESkine), a skin-associated chemokine that attracts skin-homing T lymphocytes. A recombinant soluble form of PSGL-1 (rPSGL-Ig) preferentially bound CCL27 among several chemokines tested. This interaction was abrogated by arylsulfatase treatment of rPSGL-Ig, suggesting that sulfated tyrosines play a critical role. In contrast, removal of either N-glycans or O-glycans by glycosidase treatment of rPSGL-Ig did not affect the interaction. The binding of CCL27 to a recombinant PSGL-1 synthesized in the presence of a sulfation inhibitor was lower than that produced in normal medium. Moreover, mutation of the tyrosines at the amino terminus of PSGL-1 to phenylalanine abolished the binding, further supporting the role of sulfated tyrosines in the CCL27-PSGL-1 interaction. Functionally, rPSGL-Ig reduced the chemotaxis of L1.2 cells expressing CCR10, the receptor for CCL27. In addition, the expression of human PSGL-1 on CCR10-expressing L1.2 cells resulted in reduced chemotaxis to CCL27. These findings suggest a role for PSGL-1 in regulating chemokine-mediated responses, in addition to its role as a selectin ligand."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m409868200"xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Fukuda M."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Furukawa Y."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Hirata T."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Yoshie O."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Miyasaka M."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Hieshima K."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Kannagi R."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/author | "Yang B.G."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/pages | "51775-51782"xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/title | "Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus."xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/15466853 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15466853 |
| http://purl.uniprot.org/citations/15466853 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15466853 |
| http://purl.uniprot.org/uniprot/#_Q14242-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_A0A0C4DFY0-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_B2RWM4-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_B4DHR9-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_B4DT54-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_G3V523-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |
| http://purl.uniprot.org/uniprot/#_Q5VZ77-mappedCitation-15466853 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15466853 |