| http://purl.uniprot.org/citations/15466885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15466885 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15466885 | http://www.w3.org/2000/01/rdf-schema#comment | "The 5-hydroxytryptamine type 4 receptor (5-HT4R) is involved in learning, feeding, respiratory control and gastrointestinal transit. This receptor is one of the G-protein-coupled receptors for which alternative mRNA splicing generates the most variants that differ in their C-terminal extremities. Some 5-HT4R variants (a, e and f) express canonical PDZ ligands at their C-termini. Here, we have examined whether some mouse 5-HT4R variants associate with specific sets of proteins, using a proteomic approach based on peptide-affinity chromatography, two-dimensional electrophoresis and mass spectrometry. We have identified ten proteins that interact specifically with the 5-HT4(a)R and three that only associate with the 5-HT4(e)R. Most of them are PDZ proteins. Among the proteins that associated specifically with the 5-HT4(a)R variant, NHERF greatly modified its subcellular localization. Moreover, NHERF recruited the 5-HT4(a)R to microvilli, where it localized with activated ezrin, consistent with the role of 5-HT4(a)R in cytoskeleton remodelling. The 5-HT4(a)R also interacted with both the constitutive and inducible (upon methamphetamine treatment) forms of the recently cloned sorting nexin 27 (SNX27a and b, respectively). We found that SNX27a redirected part of 5-HT4(a)R to early endosomes. The interaction of the 5-HT4R splice variants with distinct sets of PDZ proteins might specify their cellular localization as well as their signal transduction properties."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01379"xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01379"xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Hong W."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Hong W."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Barthet G."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Barthet G."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Bockaert J."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Bockaert J."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Claeysen S."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Claeysen S."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Dumuis A."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Dumuis A."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Hanson B."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Hanson B."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Joubert L."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Joubert L."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Marin P."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Marin P."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Sebben M."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/author | "Sebben M."xsd:string |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15466885 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |