| http://purl.uniprot.org/citations/15466891 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15466891 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15466891 | http://www.w3.org/2000/01/rdf-schema#comment | "The anaphase-promoting complex or cyclosome (APC/C) is a multi-subunit ubiquitin ligase that regulates the eukaryotic cell cycle. APC/C belongs to the RING finger class of ubiquitin ligases that function by interacting with a ubiquitin-conjugating enzyme (Ubc), thus inciting the Ubc to transfer ubiquitin onto a target protein. Extensive studies with APC/C in other organisms have identified several possible Ubcs that might function as partners for APC/C. This report presents phenotypic and biochemical evidence showing that, in Caenorhabditis elegans, UBC-2 interacts specifically with the APC/C. This conclusion is based on three lines of evidence: first, the RNAi phenotype of ubc-2 is indistinguishable from RNAi phenotypes of APC/C subunits; second, RNAi of ubc-2 but not other Ubcs enhances the phenotype of hypomorphic APC/C mutants; third, purified UBC-2 and APC-11, the RING finger subunit of the APC/C, show robust ubiquitination activity in in vitro assays. APC-11 interaction is specific for UBC-2 as ubiquitination is not seen when APC-11 is combined other C. elegans Ubcs. As expected from the Ubc that functions with the APC/C, ubc-2(RNAi) produces metaphase blocks in both mitotic germ cells and in meiotic divisions of post-fertilization oocytes. In addition, ubc-2(RNAi) results in two germline phenotypes that appear to be unrelated to the APC/C: an expanded transition zone indicative of a pre-pachytene meiotic arrest and endo-reduplicated oocytes indicative of a problem in ovulation or oocyte-soma interactions."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01417"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.01417"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Boyd L."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Boyd L."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Frazier T."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Frazier T."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Hota U."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Hota U."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Shakes D."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/author | "Shakes D."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/name | "J. Cell Sci."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/pages | "5427-5435"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/pages | "5427-5435"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/title | "Caenorhabditis elegans UBC-2 functions with the anaphase-promoting complex but also has other activities."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/title | "Caenorhabditis elegans UBC-2 functions with the anaphase-promoting complex but also has other activities."xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://purl.uniprot.org/core/volume | "117"xsd:string |
| http://purl.uniprot.org/citations/15466891 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15466891 |
| http://purl.uniprot.org/citations/15466891 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15466891 |