| http://purl.uniprot.org/citations/15474363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15474363 | http://www.w3.org/2000/01/rdf-schema#comment | "Gamma-secretase is a high molecular weight protein complex composed of four subunits, namely, presenilin (PS; 1 or 2), nicastrin, anterior pharynx defective-1 (Aph-1; A or B), and presenilin enhancer-2 (Pen-2), and is responsible for the cleavage of a number of type-1 transmembrane proteins. A fundamental question is whether different gamma-secretase complexes exist in vivo. We demonstrate here by in situ hybridization and by Northern and Western blotting that the gamma-secretase components are widely distributed in all tissues investigated. The expression of the different subunits seems tightly coregulated. However, some variation in the expression of the Aph-1 proteins is observed, Aph-1A being more general and abundantly distributed than Aph-1B. The previously uncharacterized rodent-specific Aph-1C mRNA is highly expressed in the kidney and testis but not in brain or other tissues, indicating some tissue specificity for the Aph-1 component of the gamma-secretase complex. Blue-native electrophoresis revealed size heterogeneity of the mature gamma-secretase complex in various tissues. Using co-immunoprecipitations and blue-native electrophoresis at endogenous protein levels, we find evidence that several independent gamma-secretase complexes can coexist in the same cell type. In conclusion, our results suggest that gamma-secretase is a heterogeneous family of protein complexes widely expressed in the adult organism."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.nbd.2004.08.002"xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Annaert W."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "De Strooper B."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Hebert S.S."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Dabrowski M."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Hartmann D."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Serneels L."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Horre K."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Dejaegere T."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/author | "Baert V."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/name | "Neurobiol Dis"xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/pages | "260-272"xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/title | "Coordinated and widespread expression of gamma-secretase in vivo: evidence for size and molecular heterogeneity."xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/15474363 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15474363 |
| http://purl.uniprot.org/citations/15474363 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15474363 |
| http://purl.uniprot.org/uniprot/P49769#attribution-CDFA5EA1B83A0A45892CE4FFAC82F430 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/15474363 |
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| http://purl.uniprot.org/uniprot/#_A0A0R4J1F2-mappedCitation-15474363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15474363 |
| http://purl.uniprot.org/uniprot/#_D3Z0X1-mappedCitation-15474363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15474363 |
| http://purl.uniprot.org/uniprot/#_A0A140LHM2-mappedCitation-15474363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15474363 |
| http://purl.uniprot.org/uniprot/#_A0A140LHQ1-mappedCitation-15474363 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15474363 |