| http://purl.uniprot.org/citations/15480750 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15480750 | http://www.w3.org/2000/01/rdf-schema#comment | "Electroneutral NaCl absorption in the intestine is mediated by parallel Na+/H+ and Cl-/HCO3-exchange. Mutations in the down-regulated in adenoma (DRA) gene cause congenital chloride diarrhoea but the transport characteristics of human DRA have not been studied in a heterologous human expression system. A N-terminal enhanced green fluorescence protein (EGFP)-tagged human DRA construct was therefore expressed stably in HEK293 cells. Cl-/HCO3-exchange was assessed by measuring intracellular pH and intracellular Cl-using fluorescent dyes. Expression of DRA resulted in the appearance of EGFP fluorescence and DRA immunoreactivity consistent with a location in the plasma membrane and possibly structures below the plasma membrane. DRA mediated electroneutral Cl-/HCO3- exchange but OH- was not transported and SO4(2-)/HCO3-exchange was minimal. In the presence of 5% CO2/HCO3-the apparent affinity of DRA for Cl-in transfected HEK cells was 23-36 mM, which is lower than that reported for rabbit ileal brush border membrane vesicles and for oocytes injected with human DRA. DRA was inhibited by 4 mM DIDS (45+/-11%), by 50 microM tenidap (71+/-8%) and by 100 microM glibenclamide (59+/-22% inhibition of HCO3-transport and 79+/-3% inhibition of Cl-transport). The effects of DIDS and tenidap were not additive to those of glibenclamide."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00424-004-1342-x"xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/author | "Gregor M."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/author | "Baisch S."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/author | "Lamprecht G."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/author | "Schoenleber E."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/name | "Pflugers Arch"xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/pages | "479-490"xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/title | "Transport properties of the human intestinal anion exchanger DRA (down-regulated in adenoma) in transfected HEK293 cells."xsd:string |
| http://purl.uniprot.org/citations/15480750 | http://purl.uniprot.org/core/volume | "449"xsd:string |
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