| http://purl.uniprot.org/citations/15480755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15480755 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15480755 | http://www.w3.org/2000/01/rdf-schema#comment | "Plastids are known to be able to synthesize their own iron-sulfur clusters, but the biochemical machinery responsible for this process is not known. In this study it is investigated whether CpNifS, the chloroplastic NifS-like cysteine desulfurase of Arabidopsis thaliana (L.) Heynh. is responsible for the release of sulfur from cysteine for the biogenesis of iron-sulfur (Fe-S) clusters in chloroplasts. Using an in vitro reconstitution assay it was found that purified CpNifS was sufficient for Fe-S cluster formation in ferredoxin in the presence of cysteine and a ferrous iron salt. Antibody-depletion experiments using stromal extract showed that CpNifS is also essential for the Fe-S cluster formation activity of chloroplast stroma. The activity of CpNifS in the stroma was 50-to 80-fold higher than that of purified CpNifS on a per-protein basis, indicating that other stromal factors cooperate in Fe-S cluster formation. When stromal extract was separated on a gel-filtration column, most of the CpNifS eluted as a dimer of 86 kDa, but a minor fraction of the stromal CpNifS eluted at a molecular weight of approx. 600 kDa, suggesting the presence of a multi-protein complex. The possible nature of the interacting proteins is discussed."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00425-004-1388-1"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00425-004-1388-1"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Ye H."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Ye H."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Pilon M."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Pilon M."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Abdel-Ghany S.E."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Abdel-Ghany S.E."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Garifullina G.F."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Garifullina G.F."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Pilon-Smits E.A."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/author | "Pilon-Smits E.A."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/name | "Planta"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/name | "Planta"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/pages | "602-608"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/pages | "602-608"xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/title | "The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin."xsd:string |
| http://purl.uniprot.org/citations/15480755 | http://purl.uniprot.org/core/title | "The chloroplast NifS-like protein of Arabidopsis thaliana is required for iron-sulfur cluster formation in ferredoxin."xsd:string |