| http://purl.uniprot.org/citations/15489421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15489421 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15489421 | http://www.w3.org/2000/01/rdf-schema#comment | "Platelet binding by Streptococcus gordonii strain M99 is mediated predominantly by the cell surface glycoprotein GspB. This adhesin consists of a putative N-terminal signal peptide, two serine-rich regions (SRR1 and SRR2), a basic region between SRR1 and SRR2, and a C-terminal cell wall anchoring domain. The glycosylation of GspB is mediated at least in part by Gly and Nss, which are encoded in the secY2A2 locus immediately downstream of gspB. This region also encodes two proteins (Gtf and Orf4) that are required for the expression of GspB but whose functions have not been delineated. In this study, we further characterized the roles of Gly, Nss, Gtf, and Orf4 by investigating the expression and glycosylation of a series of glutathione S-transferase-GspB fusion proteins in M99 and in gly, nss, gtf, and orf4 mutants. Compared with fusion proteins expressed in the wild-type background, fusion proteins expressed in the mutant strain backgrounds showed altered electrophoretic mobility. In addition, the fusion proteins formed insoluble aggregates in protoplasts of the gtf and orf4 mutants. Glycan detection and lectin blot analysis revealed that SRR1 and SRR2 were glycosylated but that the basic region was unmodified. When the fusion protein was expressed in Escherichia coli, glycosylation of this protein was observed only in the presence of both gtf and orf4. These results demonstrate that Gly, Nss, Gtf, and Orf4 are all involved in the intracellular glycosylation of SRRs. Moreover, Gtf and Orf4 are essential for glycosylation, which in turn is important for the solubility of GspB."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.186.21.7100-7111.2004"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.186.21.7100-7111.2004"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.org/dc/terms/identifier | "doi:10.1128/JB.186.21.7100-7111.2004"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Bensing B.A."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Bensing B.A."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Sullam P.M."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Sullam P.M."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Takamatsu D."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/author | "Takamatsu D."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/pages | "7100-7111"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/pages | "7100-7111"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/title | "Four proteins encoded in the gspB-secY2A2 operon of Streptococcus gordonii mediate the intracellular glycosylation of the platelet-binding protein GspB."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/title | "Four proteins encoded in the gspB-secY2A2 operon of Streptococcus gordonii mediate the intracellular glycosylation of the platelet-binding protein GspB."xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/15489421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15489421 |
| http://purl.uniprot.org/citations/15489421 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15489421 |
| http://purl.uniprot.org/citations/15489421 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15489421 |