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http://purl.uniprot.org/citations/15498567 | http://www.w3.org/2000/01/rdf-schema#comment | "Here, we produced the C-terminal truncation variants of mouse inducible heat shock protein 72 (Hsp72) to elucidate the regulatory role of the C-terminal helical lid of Hsp70 for substrate recognition. All of the truncation variants containing the substrate binding domain bound a short-length peptide substrate CLLLSAPRR. When a large mass reduced carboxymethyl alpha-lactalbumin (RCMLA) as a substrate was used in gel filtration experiment, we observed the complex formation only for the truncation variants containing the long alpha-helix C in the helical lid. However, RCMLA binding occurred even for the variants lacking alpha-helix C when their C-terminal region was anchored onto a solid phase. Together with the finding that helix C is involved in the self-association of Hsp70, our present data suggest that the C-terminal region of Hsp70 modulates the substrate recognition and its kinetics may be substrate-mass dependent."xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.febslet.2004.09.044"xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/author | "Ohno M."xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/author | "Tani F."xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/author | "Kitabatake N."xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/name | "FEBS Lett"xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/pages | "381-386"xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/title | "Role of the C-terminal region of mouse inducible Hsp72 in the recognition of peptide substrate for chaperone activity."xsd:string |
http://purl.uniprot.org/citations/15498567 | http://purl.uniprot.org/core/volume | "576"xsd:string |
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