| http://purl.uniprot.org/citations/15513933 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15513933 | http://www.w3.org/2000/01/rdf-schema#comment | "Liddle's syndrome is a genetic form of hypertension linked to Na(+) retention caused by activating mutations in the COOH terminus of the beta or gamma subunit of the epithelial sodium channel (ENaC). In this study, we used the short-circuit current (I(sc)) method to investigate the effects of deamino-8-d-arginine vasopressin (dDAVP) on Na(+) and Cl(-) fluxes in primary cultures of cortical collecting ducts (CCDs) microdissected from the kidneys of mice with Liddle's syndrome carrying a stop codon mutation, corresponding to the beta-ENaC R(566) stop mutation (L) found in the original pedigree. Compared to wild-type (+/+) CCD cells, untreated L/+ and L/L CCD cells exhibited 2.7- and 4.2-fold increases, respectively, in amiloride-sensitive (Ams) I(sc), reflecting ENaC-dependent Na(+) absorption. Short-term incubation with dDAVP caused a rapid and significant increase (approximately 2-fold) in Ams I(sc) in +/+, but not in L/+ or L/L CCD cells. In sharp contrast, dDAVP induced a greater increase in 5-nitro-2-(3-phenylpropamino)benzoate (NPPB)-inhibited apical Cl(-) currents in amiloride-treated L/L and L/+ cells than in their +/+ counterparts. I(sc) recordings performed under apical ion substituted conditions revealed that the dDAVP-stimulated apical secretion of Cl(-), which was absent in cultured CCDs lacking CFTR, was 1.8-fold greater in L/+ and 3.7-fold greater in L/L CCD cells than in their +/+ CCD counterparts. After the basal membrane had been permeabilized with nystatin and a basal-to-apical Cl(-) gradient had been imposed, dDAVP also stimulated larger Cl(-) currents across L/L and L/+ CCD layers than +/+ CCD layers. These findings demonstrate that vasopressin stimulates greater apical CFTR Cl(-) conductance in the renal CCD cells of mice with Liddle's syndrome than in wild-type mice. This effect could contribute to the enhanced NaCl reabsorption observed in the distal nephron of patients with Liddle's syndrome."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.org/dc/terms/identifier | "doi:10.1113/jphysiol.2004.077933"xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Bens M."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Schild L."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Rossier B.C."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Boulkroun S."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Teulon J."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Chang C.T."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Vandewalle A."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/author | "Hummler E."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/name | "J Physiol"xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/pages | "271-284"xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/title | "Vasopressin-stimulated CFTR Cl- currents are increased in the renal collecting duct cells of a mouse model of Liddle's syndrome."xsd:string |
| http://purl.uniprot.org/citations/15513933 | http://purl.uniprot.org/core/volume | "562"xsd:string |
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