| http://purl.uniprot.org/citations/1551888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1551888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/1551888 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/1551888 | http://www.w3.org/2000/01/rdf-schema#comment | "beta-Ketoacyl-acyl carrier protein (ACP) synthase III catalyzes the condensation of acetyl-CoA with malonyl-ACP in dissociated (Type II) fatty acid synthase systems. A synthase III mutant was used to localize the structural gene to the 24.5-min region of the Escherichia coli chromosome, and the defective synthase III allele was designated fabH1. The fabH gene was identified on a 1.3-kilobase NruI-HindIII chromosomal DNA fragment (plasmid pWO114) that complemented the enzymatic defect in fabH1 strains. The NruI-HindIII fragment was sequenced and contained a single open reading frame predicted to encode a 33,517-dalton protein with an isoelectric point of 4.85. The fabH sequence contained an Ala-Cys-Ala tripeptide characteristic of condensing enzyme active sites. A T7 expression system showed that the NruI-HindIII fragment directed the synthesis of a single 34,800-dalton protein. This protein was purified and the order of the amino-terminal 30 residues of the protein corresponded exactly to the amino acid structure predicted from the DNA sequence. The purified protein possessed both acetoacetyl-ACP synthase and acetyl-CoA:ACP transacylase activities, and cells harboring plasmid pWO114 overproduced the two activities, supporting the conclusion that a single protein carries out both reactions. Overproduction of synthase III resulted in a significant increase in shorter-chain fatty acids in the membrane phospholipids. These catalytic properties are consistent with the proposed role of synthase III in the initiation of fatty acid synthesis."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)50498-7"xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0021-9258(19)50498-7"xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Jackowski S."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Jackowski S."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Larson T.J."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Larson T.J."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Rock C.O."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Rock C.O."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Oh W."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Oh W."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Tsay J.-T."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/author | "Tsay J.-T."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/date | "1992"xsd:gYear |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/pages | "6807-6814"xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/pages | "6807-6814"xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/title | "Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/title | "Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12."xsd:string |
| http://purl.uniprot.org/citations/1551888 | http://purl.uniprot.org/core/volume | "267"xsd:string |