| http://purl.uniprot.org/citations/15541720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15541720 | http://www.w3.org/2000/01/rdf-schema#comment | "USF is a small family of basic helix-loop-helix leucine zipper (bHLH-zip) transcription factors with DNA binding specificities similar to that of the c-Myc oncoprotein. Evidence for a role of USF in growth control includes inhibition of c-Myc-dependent cellular transformation in vitro and loss of USF transcriptional activity in many cancer cell lines. However, a direct effect of USF on the tumorigenicity of an established cell line has never been demonstrated. Here, cell lines derived from rat embryo fibroblasts transformed by c-Ha-Ras and either c-Myc or E1A were used as model system to investigate the tumor suppression ability of USF. Overexpression of USF2 stimulated transcription and inhibited colony formation in c-Myc-transformed, but not E1A-transformed, fibroblasts. Stable clones expressing high USF2 levels were constructed from c-Myc-transformed fibroblasts. In two of these clones, overexpressed USF2 did not activate transcription, and there was no significant change in the transformed phenotype. In contrast, a clone that expressed transcriptionally active USF2 exhibited altered morphology and a strongly decreased ability to proliferate in semisolid medium. The ability of these cells to form tumors in nude mice was also decreased by a factor of more than 30 as compared to the parental cell line or cells overexpressing transcriptionally inactive USF2. Cotransfection assays with USF- or Myc-specific dominant-negative mutants indicated that active USF2 inhibited cellular transformation by preventing transcriptional repression by c-Myc."xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2004.08.013"xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/author | "Chen N."xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/author | "Choe C."xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/author | "Sawadogo M."xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/name | "Exp Cell Res"xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/pages | "1-10"xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/title | "Decreased tumorigenicity of c-Myc-transformed fibroblasts expressing active USF2."xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://purl.uniprot.org/core/volume | "302"xsd:string |
| http://purl.uniprot.org/citations/15541720 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15541720 |
| http://purl.uniprot.org/citations/15541720 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15541720 |
| http://purl.uniprot.org/uniprot/#_Q64705-mappedCitation-15541720 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15541720 |
| http://purl.uniprot.org/uniprot/Q64705 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/15541720 |