| http://purl.uniprot.org/citations/15542821 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15542821 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15542821 | http://www.w3.org/2000/01/rdf-schema#comment | "Prp2 is an RNA-dependent ATPase that activates the spliceosome before the first transesterification reaction of pre-mRNA splicing. Prp2 has extensive homology throughout the helicase domain characteristic of DEXD/H-box helicases and a conserved carboxyl-terminal domain also found in the spliceosomal helicases Prp16, Prp22, and Prp43. Despite the extensive homology shared by these helicases, each has a distinct, sequential role in splicing; thus, uncovering the determinants of specificity becomes crucial to the understanding of Prp2 and the other DEAH-splicing helicases. Mutations in an 11-mer near the C-terminal end of Prp2 eliminate its spliceosome binding and splicing activity. Here we show that a helicase-associated protein interacts with this domain and that this interaction contributes to the splicing process. First, a genome-wide yeast two-hybrid screen using Prp2 as bait identified Spp2, which contained a motif with glycine residues found in a number of RNA binding proteins. SPP2 was originally isolated as a genetic suppressor of a prp2 mutant. In a reciprocal screen, Spp2 specifically pulled out the C-terminal half of Prp2. Mutations in the Prp2 C-terminal 11-mer that disrupted function or spliceosome binding also disrupted Spp2 interaction. A screen of randomly mutagenized SPP2 clones identified an Spp2 protein with a mutation in the G patch that could restore interaction with Prp2 and enhanced splicing in a prp2 mutant strain. The study identifies a potential mechanism for Prp2 specificity mediated through a unique interaction with Spp2 and elucidates a role for a helicase-associated protein in the binding of a DEXD/H-box protein to the spliceosome."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.24.23.10101-10110.2004"xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.24.23.10101-10110.2004"xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Maeda A."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Maeda A."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Smith P."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Smith P."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Wei J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Wei J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Beggs J.D."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Beggs J.D."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Lin R.-J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Lin R.-J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Silverman E.J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/author | "Silverman E.J."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/name | "Mol. Cell. Biol."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/pages | "10101-10110"xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/pages | "10101-10110"xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/title | "Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing."xsd:string |
| http://purl.uniprot.org/citations/15542821 | http://purl.uniprot.org/core/title | "Interaction between a G-patch protein and a spliceosomal DEXD/H-box ATPase that is critical for splicing."xsd:string |