| http://purl.uniprot.org/citations/15557322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15557322 | http://www.w3.org/2000/01/rdf-schema#comment | "Osteopontin (OPN) is an important chemokinetic agent for several cell types. Our earlier studies have shown that its expression is essential for uridine triphosphate (UTP)-mediated migration of vascular smooth muscle cells. We demonstrated previously that the activation of an AP-1 binding site located 76 bp upstream of the transcription start in the rat OPN promoter is involved in the induction of OPN expression. In this work, using a luciferase promoter deletion assay, we identified a new region of the rat OPN promoter (-1837 to -1757) that is responsive to UTP. This region contains an NFkappaB site located at -1800 and an Ebox located at -1768. Supershift electrophoretic mobility shift assay and chromatin immunoprecipitation assays identified NFkappaB and USF-1/USF-2 as the DNA binding proteins induced by UTP, respectively, for these two sites. Using dominant negative mutants of IkappaB kinase and USF transcription factors, we confirmed that NFkappaB and USF-1/USF-2 are involved in the UTP-mediated expression of OPN. Using a pharmacological approach, we demonstrated that USF proteins are regulated by the extracellular signal-regulated kinase (ERK)1/2 pathway, just as the earlier discovered AP-1 complex, whereas NFkappaB is up-regulated through PKCdelta signals. Finally, our work suggests that the UTP-stimulated OPN expression involves a coordinate regulation of PKCdelta-NFkappaB, ERK1/2-USF, and ERK1/2/NAD(P)H oxidase AP-1 signaling pathways."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m411786200"xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Belloc I."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Desgranges C."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Potier M."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Gadeau A.P."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Renault M.A."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Dekker L.V."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Genot E."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/author | "Jalvy S."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/pages | "2708-2713"xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/title | "UTP induces osteopontin expression through a coordinate action of NFkappaB, activator protein-1, and upstream stimulatory factor in arterial smooth muscle cells."xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15557322 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15557322 |
| http://purl.uniprot.org/citations/15557322 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/15557322 |
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