| http://purl.uniprot.org/citations/15561840 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15561840 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15561840 | http://www.w3.org/2000/01/rdf-schema#comment | "As part of the SENTRY Antimicrobial Surveillance Program in 2002, five multidrug-resistant Pseudomonas aeruginosa clinical isolates were detected with metallo-beta-lactamase (MbetaL) activity. The isolates were recovered from different patients in a medical center located in Dusseldorf, Germany. The resistant determinant was isolated amplifying the region between the integrase and the aacA4 gene cassette. Sequencing revealed a novel MbetaL gene, designated bla(GIM-1). Additional analysis showed that GIM-1, comprising 250 amino acids and with a pI value of 5.4, differs in its primary sequence from that described for IMP, VIM, and SPM-1 enzymes by 39 to 43%, 28 to 31%, and 28%, respectively. The enzyme possesses unique amino acids within the major consensus sequence (HXHXD) of the MbetaL family. Kinetics analysis revealed that GIM-1 has no clear preference for any substrate and did not hydrolyze azlocillin, aztreonam, and the serine-beta-lactamase inhibitors. bla(GIM-1) was found on a 22-kb nontransferable plasmid. The new MbetaL gene was embedded in the first position of a 6-kb class 1 integron, In77, with distinct features, including an aacA4 cassette downstream of the MbetaL gene that appeared to be truncated with bla(GIM-1). The aacA4 was followed by an aadA1 gene cassette that was interrupted by a copy of the IS1394. This integron also carried an oxacillinase gene, bla(OXA-2), before the 3'-CS region. GIM-1 appears to be a unique MbetaL, which is located in a distinct integron structure, and represents the fourth subclass of mobile MbetaL enzymes to be characterized."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.org/dc/terms/identifier | "doi:10.1128/AAC.48.12.4654-4661.2004"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.org/dc/terms/identifier | "doi:10.1128/aac.48.12.4654-4661.2004"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Walsh T.R."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Walsh T.R."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Castanheira M."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Castanheira M."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Jones R.N."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Jones R.N."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Toleman M.A."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Toleman M.A."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Schmidt F.J."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/author | "Schmidt F.J."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/name | "Antimicrob. Agents Chemother."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/name | "Antimicrob Agents Chemother"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/pages | "4654-4661"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/pages | "4654-4661"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/title | "Molecular characterization of a beta-lactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/title | "Molecular characterization of a beta-lactamase gene, blaGIM-1, encoding a new subclass of metallo-beta-lactamase."xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/volume | "48"xsd:string |
| http://purl.uniprot.org/citations/15561840 | http://purl.uniprot.org/core/volume | "48"xsd:string |