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http://purl.uniprot.org/citations/15567226http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15567226http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/15567226http://www.w3.org/2000/01/rdf-schema#comment"Degenerate primers were designed for use in a culture-independent PCR screening of DNA from composite fungal communities, inhabiting residues of corn stovers and leaves. According to similarity searches and alignments amplified clone sequences affiliated with glycosyl hydrolase family 7 and glycosyl hydrolase family 45 though significant sequence divergence was observed. Glycosyl hydrolases from families 7 and 45 play a crucial role in biomass conversion to fuel ethanol. Research in this renewable energy source has two objectives: (i) To contribute to development of a renewable alternative to world's limited crude fossil oil reserves and (ii) to reduce air pollution. Amplification with 18S rDNA-specific primers revealed species within the ascomycetous orders Sordariales and Hypocreales as well as basidiomycetous order Agaricales to be present in these communities. Our study documents the value of culture-independent PCR in microbial diversity studies and could add to development of a new enzyme screening technology."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.org/dc/terms/identifier"doi:10.1016/j.mimet.2004.08.013"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.org/dc/terms/identifier"doi:10.1016/j.mimet.2004.08.013"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Jacobsen J."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Jacobsen J."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Lange L."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Lange L."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Lydolph M."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/author"Lydolph M."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/name"J. Microbiol. Methods"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/name"J Microbiol Methods"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/pages"63-71"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/pages"63-71"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/title"Culture independent PCR: an alternative enzyme discovery strategy."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/title"Culture independent PCR: an alternative enzyme discovery strategy."xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/volume"60"xsd:string
http://purl.uniprot.org/citations/15567226http://purl.uniprot.org/core/volume"60"xsd:string
http://purl.uniprot.org/citations/15567226http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15567226
http://purl.uniprot.org/citations/15567226http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/15567226
http://purl.uniprot.org/citations/15567226http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15567226
http://purl.uniprot.org/citations/15567226http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/15567226