| http://purl.uniprot.org/citations/15587270 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15587270 | http://www.w3.org/2000/01/rdf-schema#comment | "We report here knock-in of diphteria toxin A chain (dta) gene at the Ins2 locus, using the strategy previously employed to insert lacZ under control of the Ins2 promoter. Mutant Ins2(dta/+), Ins2(dta/lacZ) or Ins2(lacZ/+) mouse pups were generated by breeding and analyzed to study the effects of toxigenetic beta-cell ablation on islet development and to localize the extrapancreatic Ins2 expression site in the brain. Ins2(dta/+) and Ins2(dta/lacZ) pups developed a severe diabetic ketoacidosis and died rapidly. Histological analysis of their pancreas revealed that beta-cells completely disappeared in their islets as evidenced by loss of lacZ activity or insulin immunonostaining. beta-cell ablation did not alter the size of other islet cell populations which were normal at birth, although the glucagon-cell population was reduced by 85% at embryonic day E12.5. In the brain, comparative analysis of lacZ expression in Ins2(lacZ/+) and Ins2(dta/laZ) mice identified the choroid plexus (CP) as a major Ins2 expression site. This finding was confirmed by RT-PCR analysis of insulin transcripts in RNAs prepared from microdissected wild-type CP. Transcripts for other key beta-cell markers, with the notable exception of Pdx-1, were also found in CP RNAs. These results must revive interest in studies focused on extrapancreatic insulin gene expression."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11248-004-9587-x"xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Joshi R.L."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Deltour L."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Jami J."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Bucchini D."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Jackerott M."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/author | "Lamotte L."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/name | "Transgenic Res"xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/pages | "463-473"xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/title | "Knock-in of diphteria toxin A chain gene at Ins2 locus: effects on islet development and localization of Ins2 expression in the brain."xsd:string |
| http://purl.uniprot.org/citations/15587270 | http://purl.uniprot.org/core/volume | "13"xsd:string |
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