| http://purl.uniprot.org/citations/15590099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15590099 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15590099 | http://www.w3.org/2000/01/rdf-schema#comment | "A combined proteomic and transcriptomic analysis of Staphylococcus aureus strain N315 was performed to study a sequenced strain at the system level. Total protein and membrane protein extracts were prepared and analyzed using various proteomic workflows including: 2-DE, SDS-PAGE combined with microcapillary LC-MALDI-MS/MS, and multidimensional liquid chromatography. The presence of a protein was then correlated with its respective transcript level from S. aureus cells grown under the same conditions. Gene-expression data revealed that 97% of the 2'596 ORFs were detected during the post-exponential phase. At the protein level, 23% of these ORFs (591 proteins) were identified. Correlation of the two datasets revealed that 42% of the identified proteins (248 proteins) were amongst the top 25% of genes with highest mRNA signal intensities, and 69% of the identified proteins (406 proteins) were amongst the top 50% with the highest mRNA signal intensities. The fact that the remaining 31% of proteins were not strongly expressed at the RNA level indicates either that some low-abundance proteins were identified or that some transcripts or proteins showed extended half-lives. The most abundant classes identified with the combined proteomic and transcriptomic approach involved energy production, translational activities and nucleotide transport, reflecting an active metabolism. The simultaneous large-scale analysis of transcriptomes and proteomes enables a global and holistic view of the S. aureus biology, allowing the parallel study of multiple active events in an organism."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mimet.2004.09.017"xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.mimet.2004.09.017"xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Appel R.D."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Appel R.D."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Corthals G.L."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Corthals G.L."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Hochstrasser D.F."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Hochstrasser D.F."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Sanchez J.-C."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Sanchez J.-C."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Bento M."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Bento M."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Charbonnier Y."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Charbonnier Y."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Converset V."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Converset V."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Deshusses J.M."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Deshusses J.M."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Francois P."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Francois P."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Hoogland C."xsd:string |
| http://purl.uniprot.org/citations/15590099 | http://purl.uniprot.org/core/author | "Hoogland C."xsd:string |