| http://purl.uniprot.org/citations/15593144 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15593144 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15593144 | http://www.w3.org/2000/01/rdf-schema#comment | "Birch pollen-related food allergies are mainly associated to Bet v 1. Little is known about isoforms of Bet v 1 homologous in fruit of the Rosaceae family. We attempted to identify novel isoforms of Pru av 1, the major cherry allergen, at the cDNA and the protein level by a combination of molecular biology and proteomic tools. A cDNA library was screened with patients immunoglobulin E (IgE) and a specific hybridization probe. Edman sequencing, mass spectrometry (MS), and MS/MS were performed after detecting Pru av 1 on 2-D maps by immunoblotting using patients IgE and a monoclonal antibody. Partial amino acid sequences were completed with a polymerase chain reaction (PCR) strategy. The IgE-binding properties of the Pru av 1 spots were analyzed by 2-D blot inhibition. cDNA library analysis revealed a novel Pru av 1 isoform. MS and N-terminal sequencing confirmed the cDNA sequences at the protein level. A series of spots were confirmed as the already known Pru av 1. One spot, exclusively detected with patients sera, was identified as the novel isoform. A partial amino acid sequence detected with MS/MS was completed by PCR-cloning. The 2-D blot inhibition revealed epitope differences between the novel isoform and the previously published Pru av 1. Our data demonstrate that a synergistic combination of molecular biology and proteomics represents a powerful tool for reliable and comprehensive identification of allergen isoforms and variants. The newly identified isoform showed diverging IgE-binding properties and may be relevant for the diagnosis or therapy of cherry allergy."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200400874"xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200400874"xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Conti A."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Conti A."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Fortunato D."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Fortunato D."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Scheurer S."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Scheurer S."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Vieths S."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Vieths S."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Reuter A."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Reuter A."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Giuffrida M.G."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Giuffrida M.G."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Napolitano L."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Napolitano L."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Garoffo L.P."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/author | "Garoffo L.P."xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/15593144 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |