| http://purl.uniprot.org/citations/15595273 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15595273 | http://www.w3.org/2000/01/rdf-schema#comment | "Pit-1 is a pituitary-specific POU-domain DNA binding factor, which binds to and trans-activates promoters of growth hormone- (GH), prolactin-(PRL) and thyroid stimulating hormone beta-(TSHbeta) encoding genes. Pit-1 has been identified in several mammalian and avian species. Thyrotropin-releasing hormone (TRH) is located in the hypothalamus and it stimulates TSH, GH and PRL release from the pituitary gland. In the present study, we successfully developed a competitive RT-PCR for the detection of Pit-1 expression in the chicken pituitary, that was sensitive enough to detect picogram levels of Pit-1 mRNA. Applying this method, the effect of TRH injections on Pit-1 mRNA expression was determined in the pituitary of chick embryos and growing chicks. In both 18-day-old embryos and 10-day-old male chicks the Pit-1 mRNA expression was significantly increased following TRH injection, thereby indicating that the stimulatory effects of TRH on several pituitary hormones is mediated via its effect on Pit-1 expression. Therefore, a semi-quantitative RT-PCR method was used to detect possible changes in GH levels. TRH affected the GH mRNA levels at both developmental stages. These results, combined with the data on Pit-1 mRNA expression, indicate that Pit-1 has a role in mediating the stimulatory effects of TRH on pituitary hormones like GH."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.org/dc/terms/identifier | "doi:10.1556/avet.52.2004.4.2"xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Darras V.M."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Decuypere E."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Van der Geyten S."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Onagbesan O.M."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Van As P."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Bruggeman V."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/author | "Careghi C."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/date | "2004"xsd:gYear |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/name | "Acta Vet Hung"xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/pages | "389-402"xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/title | "Regulation of growth hormone expression by thyrotropin-releasing hormone through the pituitary-specific transcription factor Pit-1 in chicken pituitary."xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://purl.uniprot.org/core/volume | "52"xsd:string |
| http://purl.uniprot.org/citations/15595273 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/15595273 |
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