| http://purl.uniprot.org/citations/15627969 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15627969 | http://www.w3.org/2000/01/rdf-schema#comment | "In the current postgenomic era there is a growing interest in analysis of protein complexes in their native state. Here we present a novel two-dimensional separation technique for assessment of native protein complexes. The method combines native chromatography with native electrophoresis. The approach was used to study heme-binding protein complexes in murine erythroleukemia cells. The cells were metabolically labeled with [(59)Fe]-heme and cellular lysates were separated by anion-exchange chromatography. Fractions containing the (59)Fe isotope were collected, concentrated and further separated by native gel electrophoresis. A total of 13 radioactive protein bands were detected and analyzed by liquid chromatography-tandem mass spectrometry. Thirty-three individual proteins were identified and attributed to four novel multiprotein complexes representing four different 'snapshots' of cellular events involved in hemoglobin biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.200400935"xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/author | "Sutak R."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/author | "Petrak J."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/author | "Man P."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/author | "Vyoral D."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/author | "Babusiak M."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/pages | "340-350"xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/title | "Identification of heme binding protein complexes in murine erythroleukemic cells: study by a novel two-dimensional native separation -- liquid chromatography and electrophoresis."xsd:string |
| http://purl.uniprot.org/citations/15627969 | http://purl.uniprot.org/core/volume | "5"xsd:string |
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| http://purl.uniprot.org/uniprot/#_P45376-mappedCitation-15627969 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/15627969 |
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