| http://purl.uniprot.org/citations/15652348 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15652348 | http://www.w3.org/2000/01/rdf-schema#comment | "The protein dlk, encoded by the Dlk1 gene, belongs to the Notch epidermal growth factor (EGF)-like family of receptors and ligands, which participate in cell fate decisions during development. The molecular mechanisms by which dlk regulates cell differentiation remain unknown. By using the yeast two-hybrid system, we found that dlk interacts with Notch1 in a specific manner. Moreover, by using luciferase as a reporter gene under the control of a CSL/RBP-Jk/CBF-1-dependent promoter in the dlk-negative, Notch1-positive Balb/c 14 cell line, we found that addition of synthetic dlk EGF-like peptides to the culture medium or forced expression of dlk decreases endogenous Notch activity. Furthermore, the expression of the gene Hes-1, a target for Notch1 activation, diminishes in confluent Balb/c14 cells transfected with an expression construct encoding for the extracellular EGF-like region of dlk. The expression of Dlk1 and Notch1 increases in 3T3-L1 cells maintained in a confluent state for several days, which is associated with a concomitant decrease in Hes-1 expression. On the other hand, the decrease of Dlk1 expression in 3T3-L1 cells by antisense cDNA transfection is associated with an increase in Hes-1 expression. These results suggest that dlk functionally interacts in vivo with Notch1, which may lead to the regulation of differentiation processes modulated by Notch1 activation and signaling, including adipogenesis."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.yexcr.2004.10.001"xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Baladron V."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Garcia-Ramirez J.J."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Ruiz-Hidalgo M.J."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Laborda J."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Diaz-Guerra M.J."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Nueda M.L."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Bonvini E."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/author | "Gubina E."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/name | "Exp Cell Res"xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/pages | "343-359"xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/title | "dlk acts as a negative regulator of Notch1 activation through interactions with specific EGF-like repeats."xsd:string |
| http://purl.uniprot.org/citations/15652348 | http://purl.uniprot.org/core/volume | "303"xsd:string |
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