| http://purl.uniprot.org/citations/15654081 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15654081 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/15654081 | http://www.w3.org/2000/01/rdf-schema#comment | "Monoamine oxidase catalyzes the oxidative deamination of a number of neurotransmitters. A deficiency in monoamine oxidase A results in aggressive behavior in both humans and mice. Studies on the regulation of monoamine oxidase A gene expression have shown that the Sp1 family is important for monoamine oxidase A expression. To search for novel transcription factors, the sequences of three Sp1 sites in the monoamine oxidase A core promoter were used in the yeast one-hybrid system to screen a human cDNA library. A novel repressor, R1 (RAM2), has been cloned. The R1 cDNA encodes a protein with 454 amino acids and an open reading frame at the 5'-end. The transfection of R1 in a human neuroblastoma cell line, SK-N-BE (2)-C, inhibited the monoamine oxidase A promoter and enzymatic activity. The degree of inhibition of monoamine oxidase A by R1 correlated with the level of R1 protein expression. R1 was also found to repress monoamine oxidase A promoter activity within a natural chromatin environment. A gel-shift assay indicated that the endogenous R1 protein in SK-N-BE (2)-C cells interacted with the R1 binding sequence. R1 also bound directly to the natural monoamine oxidase A promoter in vivo as shown by chromatin immunoprecipitation assay. Immunocytochemical analysis showed that R1 was expressed in both cytosol and nucleus, which suggested a role for R1 in transcriptional regulation. Northern blot analysis revealed the presence of endogenous R1 mRNA in human brain and peripheral tissues. Taken together, this study shows that R1 is a novel repressor that inhibits monoamine oxidase A gene expression."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m410033200"xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m410033200"xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Chen G."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Chen K."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Choi S.H."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Choi S.H."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Shih J.C."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Shih J.C."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Ou X.-M."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/author | "Ou X.-M."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/date | "2005"xsd:gYear |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/pages | "11552-11559"xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/pages | "11552-11559"xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/title | "R1, a novel repressor of the human monoamine oxidase A."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/title | "R1, a novel repressor of the human monoamine oxidase A."xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/volume | "280"xsd:string |
| http://purl.uniprot.org/citations/15654081 | http://purl.uniprot.org/core/volume | "280"xsd:string |